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차세대 염기서열 분석법을 이용한 15개 상염색체 STR의 염기서열 생성 및 유전자형 분석

Other Titles
 Sequence Generation and Genotyping of 15 Autosomal STR Markers Using Next Generation Sequencing 
Authors
 김은혜  ;  정상은  ;  신경진  ;  양우익  ;  양인석 
Citation
 Korean Journal of Legal Medicine (대한법의학회지), Vol.38(2) : 48-58, 2014 
Journal Title
 Korean Journal of Legal Medicine (대한법의학회지) 
ISSN
 2383-5702 
Issue Date
2014
Keywords
Short tandem repeat ; Next generation sequencing ; Repeat structure ; Sequence variation ; Mixture
Abstract
Recently, next generation sequencing (NGS) has received attention as the ultimate genotyping method to overcome the limitations of capillary electrophoresis (CE) based short tandem repeat (STR) analysis, such as the limited number of STR loci that can be measured simultaneously using fluorescent-labeled primers and the maximum size of STR amplicons. In this study, we analyzed 15 autosomal STR markers via the NGS method and evaluated their effectiveness in STR analysis. Using male and female standard DNA as single-sources and their 1:1 mixture, we sequentially generated sample amplicons by the multiplex polymerase chain reaction (PCR) method, constructed DNA libraries by ligation of adapters with a multiplex identifier (MID), and sequenced DNA using the Roche GS Junior Platform. Sequencing data for each sample were analyzed via alignment with pre-built reference sequences. Most STR alleles could be determined by applying a coverage threshold of 20% for the two single-sources and 10% for the 1:1 mixture. The structure of the STR in each allele was accurately determined by examining the sequences of the target STR region. The mixture ratio of the mixed sample was estimated by analyzing the coverage ratios between assigned alleles at each locus and the reference/variant ratios from the observed sequence variations. In conclusion, the experimental method used in this study allowed the successful generation of NGS data. In addition, the NGS data analysis protocol enables accurate STR allele call and repeat structure determination at each locus. Therefore, this approach using the NGS system will be helpful to interpret and analysis the STR profiles from singe-source and even mixed samples in forensic investigation.
Files in This Item:
T201405537.pdf Download
DOI
10.7580/kjlm.2014.38.2.48
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Kim, Eun Hye(김은혜)
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Yang, In Seok(양인석) ORCID logo https://orcid.org/0000-0001-5224-2587
Jung, Sang Eun(정상은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138731
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