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Helicobacter pylori induces cell migration and invasion through casein kinase 2 in gastric epithelial cells.

Authors
 Yeo Song Lee  ;  Do Yeon Lee  ;  Da Yeon Yu  ;  Shin Kim  ;  Yong Chan Lee 
Citation
 HELICOBACTER, Vol.19(6) : 465-475, 2014 
Journal Title
 HELICOBACTER 
ISSN
 1083-4389 
Issue Date
2014
MeSH
Antigens, Bacterial/genetics ; Antigens, Bacterial/metabolism* ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism* ; Casein Kinase II/genetics ; Casein Kinase II/metabolism* ; Cell Movement ; Epithelial Cells/cytology ; Epithelial Cells/enzymology* ; Epithelial Cells/microbiology ; Gastric Mucosa/cytology ; Gastric Mucosa/enzymology ; Gastric Mucosa/microbiology ; Helicobacter Infections/enzymology* ; Helicobacter Infections/genetics ; Helicobacter Infections/microbiology ; Helicobacter Infections/physiopathology ; Helicobacter pylori/genetics ; Helicobacter pylori/metabolism* ; Humans ; Signal Transduction
Keywords
Helicobacter pylori ; casein kinase 2 ; gastric epithelial cells ; invasion ; migration ; α-/β-catenin
Abstract
BACKGROUND: Chronic infection with Helicobacter pylori (H. pylori) is causally linked with gastric carcinogenesis. Virulent H. pylori strains deliver bacterial CagA into gastric epithelial cells. Induction of high motility and an elongated phenotype is considered to be CagA-dependent process. Casein kinase 2 plays a critical role in carcinogenesis through signaling pathways related to the epithelial mesenchymal transition. This study was aimed to investigate the effect of H. pylori infection on the casein kinase 2-mediated migration and invasion in gastric epithelial cells. MATERIALS AND METHODS: AGS or MKN28 cells as human gastric epithelial cells and H. pylori strains Hp60190 (ATCC 49503, CagA(+)) and Hp8822 (CagA(-)) were used. Cells were infected with H. pylori at multiplicity of infection of 100 : 1 for various times. We measured in vitro kinase assay to examine casein kinase 2 activity and performed immunofluorescent staining to observe E-cadherin complex. We also examined β-catenin transactivation through promoter assay and MMP7 expression by real-time PCR and ELISA. RESULTS: H. pylori upregulates casein kinase 2 activity and inhibition of casein kinase 2 in H. pylori-infected cells profoundly suppressed cell invasiveness and motility. We confirmed that casein kinase 2 mediates membranous α-catenin depletion through dissociation of the α-/β-catenin complex in H. pylori-infected cells. We also found that H. pylori induces β-catenin nuclear translocation and increases MMP7 expressions mediated through casein kinase 2. CONCLUSIONS: We show for the first time that CagA(+) H. pylori upregulates cellular invasiveness and motility through casein kinase 2. The demonstration of a mechanistic interplay between H. pylori and casein kinase 2 provides important insights into the role of CagA(+) H. pylori in the gastric cancer invasion and metastasis.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/hel.12144/abstract
DOI
10.1111/hel.12144
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Shin(김신)
Yu, Da Yeon(유다연)
Lee, Do Yeon(이도연)
Lee, Yong Sang(이용상) ORCID logo https://orcid.org/0000-0002-8234-8718
Lee, Yong Chan(이용찬) ORCID logo https://orcid.org/0000-0001-8800-6906
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138526
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