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Positive control for cytotoxicity evaluation of dental vinyl polysiloxane impression materials using sodium lauryl sulfate

 Jae-Sung Kwon  ;  Sang-Bae Lee  ;  Kwang-Mahn Kim  ;  Kyoung-Nam Kim 
 ACTA ODONTOLOGICA SCANDINAVICA, Vol.72(8) : 618-622, 2014 
Journal Title
Issue Date
Animals ; Cell Line ; Chromatography, Ion Exchange ; Cricetinae ; Dental Impression Materials/analysis ; Dental Impression Materials/toxicity* ; Fibroblasts/drug effects ; Materials Testing ; Mesocricetus ; Mice ; Mouth Mucosa/drug effects ; Polyvinyls/analysis ; Polyvinyls/toxicity* ; Siloxanes/analysis ; Siloxanes/toxicity* ; Sodium Dodecyl Sulfate/analysis ; Sodium Dodecyl Sulfate/toxicity* ; Sulfates/analysis ; Surface-Active Agents/analysis ; Surface-Active Agents/toxicity* ; Time Factors
biocompatibility ; cytotoxicity ; elastomeric impression materials ; sodium lauryl sulfate ; vinyl polysiloxane
Objectives. Vinyl polysiloxane (VPS) is elastomeric dental impression material which, despite having very few reports of adverse reactions, has shown high levels of cytotoxicity that is difficult to be interpreted without referencing to the positive control material. Therefore, in this study, positive control VPS was developed using sodium lauryl sulfate (SLS) for the reference of cytotoxicity test. Materials and methods. The positive control VPS with SLS was formed with a different proportion of SLS (0, 1, 2, 4, 8 and 16 wt%) added to the base. The cytotoxicity test was then carried out using the extractions or dilutions of the extractions from each of the test samples using murine fibroblast cells (L929). Results. The final product of positive control VPS behaved similar to commercially available VPS; being initially liquid-like and then becoming rubber-like. Ion chromatography showed that the level of SLS released from the product increased as the proportion of added SLS increased, consequently resulting in an increased level of cytotoxicity. Also, the commercially available VPS was less cytotoxic than the positive control VPS with more or equal to 2 wt% of SLS. However, even the VPS with the highest SLS (16 wt%) did not cause oral mucosa irritation during the animal study. Conclusions. The positive control VPS was successfully produced using SLS, which will be useful in terms of providing references during in vitro cytotoxicity testing.
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2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
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