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A comparative study of three cytotoxicity test methods for nanomaterials using sodium lauryl sulfate.

Authors
 Jae Sung Kwon  ;  Kwang Mahn Kim  ;  Kyoung Nam Kim 
Citation
 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, Vol.14(10) : 8043-8047, 2014 
Journal Title
 JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 
ISSN
 1533-4880 
Issue Date
2014
MeSH
Animals ; Cell Line ; Colorimetry ; Ethidium/analogs & derivatives ; Ethidium/chemistry ; Fibroblasts/cytology ; Fibroblasts/drug effects ; Fluoresceins/chemistry ; Mice ; Nanostructures/chemistry ; Nanostructures/toxicity* ; Sodium Dodecyl Sulfate/chemistry* ; Solubility ; Staining and Labeling ; Toxicity Tests/methods* ; Trypan Blue/chemistry ; Water/chemistry
Abstract
The biocompatibility evaluation of nanomaterials is essential for their medical diagnostic and therapeutic usage, where a cytotoxicity test is the simplest form of biocompatibility evaluation. Three methods have been commonly used in previous studies for the cytotoxicity testing of nanomaterials: trypan blue exclusion, colorimetric assay using water soluble tetrazolium (WST), and imaging under a microscope following calcein AM/ethidium homodimer-1 staining. However, there has yet to be a study to compare each method. Therefore, in this study three methods were compared using the standard reference material of sodium lauryl sulfate (SLS). Each method of the cytotoxicity test was carried out using mouse fibroblasts of L-929 exposed to different concentrations of SLS. Compared to the gold standard trypan blue exclusion test, both colorimetric assay using water soluble tetrazolium (WST) and imaging under microscope with calcein AM/ethidium homodimer-1 staining showed results that were not statistically different. Also, each method exhibited various advantages and disadvantages, which included the need of equipment, time taken for the experiment, and provision of additional information such as cell morphology. Therefore, this study concludes that all three methods of cytotoxicity testing may be valid, though careful consideration will be needed when selecting tests with regard to time, finances, and the amount of information required by the researcher(s).
Full Text
http://www.ingentaconnect.com/content/asp/jnn/2014/00000014/00000010/art00125
DOI
10.1166/jnn.2014.9415
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138265
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