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Nonmucoid conversion of mucoid Pseudomonas aeruginosa induced by sulfate-stimulated growth.

Authors
 Kyung Bae Min  ;  Kang Mu Lee  ;  Young Taek Oh  ;  Sang Sun Yoon 
Citation
 FEMS Microbiology Letters, Vol.360(2) : 157-166, 2014 
Journal Title
 FEMS Microbiology Letters 
ISSN
 0378-1097 
Issue Date
2014
MeSH
Alginates/metabolism* ; Artificial Gene Fusion ; Gene Expression Profiling ; Genes, Reporter ; Glucuronic Acid/metabolism ; Hexuronic Acids/metabolism ; Humans ; Pseudomonas aeruginosa/cytology ; Pseudomonas aeruginosa/drug effects* ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/metabolism* ; Sulfates/metabolism*
Keywords
Pseudomonas aeruginosa ; alginate ; mucoid ; nonmucoid reversion ; sulfate
Abstract
Alginate-overproducing mucoid Pseudomonas aeruginosa, responsible for chronic airway infections in cystic fibrosis (CF) patients, is resistant to antibiotic treatments and host immune clearance. In this study, we performed a phenotype microarray screen and identified sulfate ion as a molecule that can suppress alginate production. When a mucoid P. aeruginosa strain CM21 and additional mucoid isolates were grown with 5% sodium sulfate, significantly decreased levels of alginate were produced. Suppression of alginate production was also induced by other sulfate salts. Expression of a reporter gene fused to the algD promoter was considerably decreased when grown with sulfate. Furthermore, bacterial cell shape was abnormally altered in CM21, but not in PAO1, a prototype nonmucoid strain, suggesting that sulfate-stimulated cell shape change is associated with transcriptional suppression of the alginate operon. Finally, a CM21 lpxC mutant defective in lipid A biosynthesis continued to produce alginate and maintained the correct cell shape when grown with sulfate. These results suggest a potential involvement of lipoploysaccharide biosynthesis in the sulfate-induced reversion to nonmucoid phenotype. This study proposes a novel strategy that can be potentially applied to treat persistent infection by recalcitrant mucoid P. aeruginosa.
Files in This Item:
T201404151.pdf Download
DOI
10.1111/1574-6968.12600
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
Lee, Kang Mu(이강무) ORCID logo https://orcid.org/0000-0001-7414-5921
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138228
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