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Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas

Authors
 YOUNG-JIN PARK  ;  EUN-KYOUNG KIM  ;  SOOK MOON  ;  DOO-PYO HONG  ;  JUNG YOON BAE  ;  JIN KIM 
Citation
 Anticancer Research, Vol.34(11) : 6389-6395, 2014 
Journal Title
 Anticancer Research 
ISSN
 0250-7005 
Issue Date
2014
Keywords
Human telomerase reverse transcriptase (hTERT) ; immortal oral keratinocytes ; multi-step process ; squamous cell carcinoma
Abstract
BACKGROUND/AIM: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. MATERIALS AND METHODS: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. RESULTS: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. CONCLUSION: Targeting hTERT may lead to novel therapeutic approaches.
Full Text
http://ar.iiarjournals.org/content/34/11/6389.long
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun Kyoung(김은경)
Kim, Jin(김진)
Park, Young Jin(박영진)
Bae, Jung Yoon(배정윤) ORCID logo https://orcid.org/0000-0001-8342-6987
Hong, Doo Pyo(홍두표)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138225
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