Studies on the rapid and competitive enzyme-linked immunosorbent assay for the detection of thyroxine (T4) in human sera
Other Titles
경쟁적 효소면역 측정법을 이용한 환자 혈청 내 Thyroxine (T₄)의 검출
Authors
박상욱
Issue Date
1999
Description
The Graduate School Yonsei University/석사
Abstract
[한글]
Thyroxine (3,5,3',5'-L-tetraiodothyronine: T^^4) is the most commonly measured
thyroid hormone for the diagnosis of various thyroid disorders. T^^4 is synthesized
within the acinar cell of the thyroid gland, then it is coupled with oxidized
iodine in the blood, and then the T? complected with thyroglobulin which is already
produced, is released into the blood stream. Greater than 99.9% of 74 transported
through the blood stream is bound to plasma proteins, of which the maior binding
protein is thyroxine binding globulin (TBG). This hormone is also important for
growth development and sexual maturation of growing organisms, Although
radioimmunoassay (RIA) is still considered as the reference technique for the
measurement of 74 it is generally regarded that RIA has its primary disadventages
in handling the wastes and controling the human and material resources. Therefore,
establishment of enzyme-linked immunosorbent assay (ELISA) has of great
significance. To verify the usefulness of our enzyme immunoassay, we have obtained
the standard dose response curve of 74 in patient's sera which is inversely
proportional to the amount of horseradish peroxidase (HRP) conjugated monoclonal
antibody of 74bound to the wells, The correlation coefficient (r) between the ELISA
and chemiluminescent assay was 0.444 (n=38). Thus we have investigated the
establishment of rapid and sensitive competitive ELISA assay method for detection
of 74 in patient's sera,
[영문]
Thyroxine (3,5,3',5'-L-tetraiodothyronine: T^^4) is the most commonly measured thyroid hormone for the diagnosis of various thyroid disorders. T^^4 is synthesized within the acinar cell of the thyroid gland, then it is coupled with oxidized iodine in the blood, and then the T? complected with thyroglobulin which is already produced, is released into the blood stream. Greater than 99.9% of 74 transported through the blood stream is bound to plasma proteins, of which the maior binding protein is thyroxine binding globulin (TBG). This hormone is also important for growth development and sexual maturation of growing organisms, Although
radioimmunoassay (RIA) is still considered as the reference technique for the measurement of 74 it is generally regarded that RIA has its primary disadventages in handling the wastes and controling the human and material resources. Therefore, establishment of enzyme-linked immunosorbent assay (ELISA) has of great significance. To verify the usefulness of our enzyme immunoassay, we have obtained the standard dose response curve of 74 in patient's sera which is inversely proportional to the amount of horseradish peroxidase (HRP) conjugated monoclonal antibody of 74bound to the wells, The correlation coefficient (r) between the ELISA and chemiluminescent assay was 0.444 (n=38). Thus we have investigated the establishment of rapid and sensitive competitive ELISA assay method for detection of 74 in patient's sera,