Studies on the rapid and competitive enzyme-linked immunosorbent assay for the detection of thyroxine (T4) in human sera

Abstract

[한글]

Thyroxine (3,5,3',5'-L-tetraiodothyronine: T^^4) is the most commonly measured

thyroid hormone for the diagnosis of various thyroid disorders. T^^4 is synthesized

within the acinar cell of the thyroid gland, then it is coupled with oxidized

iodine in the blood, and then the T? complected with thyroglobulin which is already

produced, is released into the blood stream. Greater than 99.9% of 74 transported

through the blood stream is bound to plasma proteins, of which the maior binding

protein is thyroxine binding globulin (TBG). This hormone is also important for

growth development and sexual maturation of growing organisms, Although

radioimmunoassay (RIA) is still considered as the reference technique for the

measurement of 74 it is generally regarded that RIA has its primary disadventages

in handling the wastes and controling the human and material resources. Therefore,

establishment of enzyme-linked immunosorbent assay (ELISA) has of great

significance. To verify the usefulness of our enzyme immunoassay, we have obtained

the standard dose response curve of 74 in patient's sera which is inversely

proportional to the amount of horseradish peroxidase (HRP) conjugated monoclonal

antibody of 74bound to the wells, The correlation coefficient (r) between the ELISA

and chemiluminescent assay was 0.444 (n=38). Thus we have investigated the

establishment of rapid and sensitive competitive ELISA assay method for detection

of 74 in patient's sera,

[영문]

Thyroxine (3,5,3',5'-L-tetraiodothyronine: T^^4) is the most commonly measured thyroid hormone for the diagnosis of various thyroid disorders. T^^4 is synthesized within the acinar cell of the thyroid gland, then it is coupled with oxidized iodine in the blood, and then the T? complected with thyroglobulin which is already produced, is released into the blood stream. Greater than 99.9% of 74 transported through the blood stream is bound to plasma proteins, of which the maior binding protein is thyroxine binding globulin (TBG). This hormone is also important for growth development and sexual maturation of growing organisms, Although

radioimmunoassay (RIA) is still considered as the reference technique for the measurement of 74 it is generally regarded that RIA has its primary disadventages in handling the wastes and controling the human and material resources. Therefore, establishment of enzyme-linked immunosorbent assay (ELISA) has of great significance. To verify the usefulness of our enzyme immunoassay, we have obtained the standard dose response curve of 74 in patient's sera which is inversely proportional to the amount of horseradish peroxidase (HRP) conjugated monoclonal antibody of 74bound to the wells, The correlation coefficient (r) between the ELISA and chemiluminescent assay was 0.444 (n=38). Thus we have investigated the establishment of rapid and sensitive competitive ELISA assay method for detection of 74 in patient's sera,