Evaluation of rat periodontal ligament cell viability after hypothermic preservation under high hydrostatic pressure
Other Titles
저온고압 보관시 쥐치주인대세포의 활성도의 평가
Authors
이윤
Issue Date
2012
Description
Dept. of Dentistry/박사
Abstract
The purpose of this study was to find the ideal hypothermic condition for tooth storage by evaluating the rat periodontal ligament cell viability after two-day storage under different pressures and temperatures using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and caspase-3 fluorometric assay. The first and second maxillary molars of 4-week-old Sprague-Dawley female rats were extracted and stored under different hydrostatic pressure (no additional pressure, 2MPa, 5MPa, 20MPa, and 30MPa) and temperature (-2°C, -1°C, 0°C, and 4°C) for 2 days. Teeth immediately after extraction were used as control. After 2 days, MTT assay and caspase-3 fluorometric assay were performed to evaluate the periodontal ligament cell viability and apoptosis rate. For statistical analysis, one-way analysis of variance was used, and Tukey analysis was used for post-hoc comparison. According to MTT assay results, at all temperatures the 2MPa group had greater rat periodontal ligament cell viability than at other pressures, and at -2°C, -1°C, and 0°C, the cell viability was significantly higher at 2MPa than at no additional pressure or higher hydrostatic pressure (p<0.05). According to caspase-3 assay results, the rate of apoptosis was significantly higher in the 20MPa and 30MPa groups (p<0.05), but other groups were not significantly different from the immediate control group (p>0.05). Combining both assays, the 2MPa groups showed the highest cell viability and the lowest caspase-3 activity compared to no additional pressure or higher hydrostatic pressure groups. Within the 2MPa group, 0°C, -1°C and 4°C groups showed no statistical differences from the immediate control group.