수종 실험동물 다형핵백혈구의 candida albicans에 대한 탐식능과 혈청성분과의 유관성에 관한 연구

Abstract

[영문]

[한글]

Candida albicans에 대한 다형핵백혈구의 탐식작용은 세포내의 peroxidase system에 의

하여, H^^2O^^2에 의하여, 또한 H^^2O^^2와 lysosomal protein등에 의하여 탐식능 및 세

포내 살균현상이 나타난다. 그러나 이같은 세포질내의 효소들의 결핍이나 작용억제현상은

탐식능의 저하를 나타낸다고 알려져 있다.

이에 저자는 수종의 실험동물 및 사람에서의 Candida albicans 감염에 대한 정상균주

및 자외선조사균주의 차이를 관찰하고 다형핵백혈구에 의한 탐식능 및 살균능의 관계를

비교하고 동시에 이의 각 실험동물간 혈청인자의 차이에 따른 영향을 비교연구하여 다음

과 같은 결론을 얻었다.

1. LD^^50(lethal dose 50)을 통한 Candida albicans의 정상균주와 자외선조사균주간의

생쥐에 대한 병원성은 정상균주의 LD^^5o이 1㎖당 2.5×10**5 세포인데 반하여 자외선조

사균주의 LO^^50은 1㎖당 5×10**7 세포에서도 관찰할 수 없어 정상균주 보다 자외선조사

균주의 병원성이 감소함을 볼 수 있었다.

2. 정상균주에 대한 다형핵백혈구의 탐식능은 실험동물의 종류 및 혈청처리에 따라 사

람에서는 정상혈청: 89.2 0.7%, 열처리비동화혈청: 56.2 0.5 %, HBSS: 35.3 0.7%이

었고 가토에서는 90.7 0.5%, 67.8 0.8%, 31.2 0.8 %. 백서는 87.3 0.5 %, 65.5

0.7%, 43.3 0.7%, 생쥐는 93.3 0.8%, 55.2 0.7%, 32.4 0.7%이었다.

3. 자외선조사균주에 대한 다형핵백혈구의 탐식능은 사람에서는 정상혈청 : 91.1 0.5

%,열처러비동화혈청 : 58.3 0.4%, HBSS : 27.3 0.5%, 가토에서는 96.2 0.7%,71.3

0.6%, 26.5 0.7%, 백서는 97.2 0.4%, 64.2 0.9%, 38.4 0.8%, 생쥐에서는 95.5

0.5%, 55.8 0.2%, 30.5 0.8%로서 자외선조사균주나 정상균주에 대한 다형핵백혈구 탐

식능에는 큰 차이를 볼 수 없었으나 혈청을 열처리비동화시킨 경우와 혈청대신 HBSS를 첨

가한 경우는 정상균주 및 자외선조사균주 공히 정상혈청 첨가시 보다 탐식능의 감소를 보

였다.

4. 동종혈청 과 이종혈청시의 다형핵백혈구의 탐식능은 별다른 차이가 없었다.

5. 세포내 살균능은 탐식능에 비하여 현저히 감소되어 백서의 경우 52.4 0.9%, 가토

의 경우 43.7 0.2%, 사람의 경우 32.4 0.7%, 생쥐의 경우 23.6 0.1% 순 이었다.

6. 용혈50%에 의한 보체값은 사람의 경우 28.1 unit, 백서 23.1 unit, 가토 21.4 unit,

생쥐는 가장 낮아서 1.7 unit 순 이었다.

7. 배관 (germ tube) 형성능은 정상균주와 자외선조사균주간에 는 커다란 차이가 있어

정상균주에서의 배관형성능이 3배이상 높았으며 이중 가토혈청에서의 배관형성능이 정상

균주: 91.5%, 자외선조사균주 29.7%로서 가장 높았고, 벡서혈청에서 40.7%, ll.3%로서 가

장 낮았다.

8. 정상혈청 및 면역혈청에서 모두 응집 (clumping)현상을 관찰할 수 있었으나 면역혈

청에서는 응집감소현상을 보였다. 응집현상증가시에는 배관의 증가와, 아울러 세포표면에

특이물질의 집착을 볼 수 있었으나 응집감소현상시에는 이를 관찰하기 힘들었다.

THE RELATIONSHIP BETWEEN SERUM COMPONENT AND PHAGOCYTIC ACTIVITY TO CANDIDA

ALBICANS BY POLYMORPHONUCLEAR LEUKOCYTES IN EXPERIMENTAL ANIMALS AND HUMAN

Young Il Kim D.D.S., M.S.0.

Department of Dental Science, Graduate School , Yonsei University

(Directed by Prof. Eui W. Lee, D.D.S., M.S.D., Ph.D.)

The author studied the phagocytic and intracellular klling activity between the

normal strain and the ultraviolet-ray irradiated strain of Candida albicans by

polymorphonuclear leukocytes derived from various laboratory animals, and at the

same time, investigated how the serum factors of each laboratory animal influence

the above-mentioned activities.

The results were as follows;

1. The lethal dose 50 (LD^50) of normal Candida albicans in mice were 2.5 ×

10**5 cells/㎖, but could not be observed in the ultraviolet-ray irradiated Candida

albicans even with 5×10**7 cells/㎖. It demostrates that the virulence is

decreased in the ultraviolet-ray irradiated strains.

2. The phagocytic index of normal strains ofCandida albicans by polymorphonuclear

leukocytes from laboratory animals and human mixed with homologous sera were 89.2

0.7% in the normal human sera, 56.2 0.5% in the heat-inactivated human sera, and

35.3 0.7% in HBSS. As for rabbits, it was 90.7 0.5% in the normal sera, 67.8

0.8% in the heat-inactivated sera, and 31.2 0.8% in HBSS. As for rats, it was

87.3 0.5% in the normal sera, 65.5 0.7% in the heat-inactivated sera, and 43.3

0.7% in HBSS. And as for mice, it was 93.3 0.8% in the nonmal sera, 55.2 0.7%

in the heat-inactivated sera, and 32.4 0.7 in HBSS.

3. The phagocytic index of ultraviolet-ray irradiated Candida albicans by

polymorphonuclear leukocytes form laboratory amimals and humun mixed with

homologous sera were 91.1 0.5% in the normal human sera, 58.3 0.4%, in the

heat-inactivated human sera, and 27.3 0.5 in HBSS. As for rabbits, it was 96.2

0.7%, 71.3 0.6%, and 26.5 0.7%. As for rats, it was 97.2 0.4%, 64.2 0.9%,

and 38,4 0.8%. And an for mice, it was 95.5 0.5%, 55.8 0.2%, and 30.5 0.8%.

There was no significant difference between phagocytic effects of the normal

strains and ultraviolet-ray irradiated strains of Candida albicans by

polymorphonuclear leukocytes, however, the phagocytic effects were decreased when

the sera were heat-inactivated or when HBSS were added in place of the sera.

4. No significant difference in the phagocytic effects was observed between the

homologous sera and heterologous sera.

5. The intracellular killing index of Candida albicans by polymorphonuclear

leukocytes mixed with the mormal homologous sera from human and lavoratory animals

showed marked decrease in comparison with the phagocytic index. The intracellular

killing index was 52.4 0.9% with the rat PMN cells, 43.7% 0.2% with the rabbit

PMN cells, 32.4% 0.7% with the human PMN cells, and 23.6 0.1% with the mouse

PMN cells.

6. The complement titers of the sera from the laboratory animals and human by

CH^^50 were 28.1 unit in rat sera, 21.4 unit in rbbit sera, 23.1 unit in rat sera,

and 1.7 unit in mouse sera.

7. The germ tube formation activity of Candida albicans in various sera from

laboratory animals showed marked differences between the normal strains and the

ultraviolet-ray irradiated strains. It was markedly higher in the normal strains.

The highest was 91.5% in the normal strains treated with the rabbit sera, and

lowest was 40.7% in the normal strains treated with the rat sera.

8. The clumping phenomena could be observed both in the normal sera and in the

heat-inactivated sera, however, it showed a decreasing tendency in the immunized

sera. It was also observed that the germ tube production increased with the

increase of clumping phenomena, and at the same time, they showed some specific

materials adhered to the cell surface when the clumping increased, but none such

materials were seen when the clumping decreased.