亞黃酸가스가 白鼠組織의 Lactic Dehydrogenase-isozyme에 미치는 影響

Abstract

[영문]

[한글]

Effects of Sulfur Dioxide on Lactic Dehydrogenase-Isozyme

Chung, Yong

Department of Public Health, Graduate School, Yonsei University

(Directed by Associate Professor Kwon, Sook Pyo)

Alterations of H-and M-isozymes of Lactic Dehydrogenase(LDH) were observed in the

various tissues after exposing the rats to 50ppm and 250ppm of sulfur dioxide.

These isozyme of the respective tissues were separated by

Diethylaminoethyl(DEAE)-cellulose from the tissue homogenates of brain, heart, lung

and muscle, presenting the activities by rate of reduction of

nicotinamide-adenine-dicucleotide(NAD**+).

Pure LDH and the coenzyme (NAD**+) were directly treated with sulfur dioxide in

vitro in order to find out the direct effect to sulfur dioxide on LDH and NAD**+

and the results were as follows.

1. In the normal tissues, the H-isozyme activity was dominant in the brain and

heart, and the M-isozyme in the muscle.

2. In the lung tissue of normal rats, there was no difference between the

activity of H-and M-type of LDH.

3. When rats inhale sulfur dioxide gas in concentration of 50ppm and 250ppm, it

appeared that the H-type tend to be suppressed in aerobic tissues and the M-type in

anaerobic tissues.

4. In the lung tissue exposed to sulfur dioxide, both the LDH activities were

suppressed.

5. It seems that LDH and the coenzyme (NAD**+) are not directly affected by

exposing in sulfur dioxide gas.