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Cytotoxicity test of dentin bonding agents using dentin substitutes in a dentin barrier test

Other Titles
 상아질 격리 시험에서 대체막을 사용한 상아질 접착제의 세포 독성 평가 
Authors
 김미주 
Issue Date
2014
Description
Dept. of Dentistry/박사
Abstract
The aim of this study was to compare the experimental results of dentin barrier test using dentin substitutes – millipore filters or polyurethane disc – with those using natural bovine dentin in order to find a proper standard barrier and perform the cytotoxicity test of dentin bonding agents by selected substitute.The three-dimensional cells attached to dentin disc or millipore filters as the dentin substitute were tested in a dentin barrier test by perfusion. MTT assay was performed as an evaluation method for the cell survival rate. The cytotoxicity test of serial phenol dilution by bovine dentin disc was done to determine a standard toxic material, and the test of this proper phenol by various millipore combinations was performed to find the suitable dentin substitute. Also, the cytotoxicity test of commercial bonding agents was performed by this standardized substitute. The cell viability was expressed as percentages of untreated group. The cytotoxicity test by polyurethane discs with monolayer cells was performed as the same way by that of the millipore filters. Phenol concentration of 0.05 % was selected as the standard toxic material. The different combinations of millipore filters – two sheets of 0.45 μm, two sheets of 0.22 μm, and filter combination of 0.65 μm, 0.45 μm, and 0.22 μm – showed similar cytotoxicity to bovine dentin discs by 0.05 % phenol (P > 0.05). The millipore combination of 0.65 μm, 0.45 μm, and 0.22 μm that had the most structural similarity to dentin discs was used as the substitute for cytotoxicity test of bonding agents. The toxic level of Adper Prompt L-Pop ® using the selected substitute was significantly the highest among four kinds of dentin bonding agents (P < 0.05). Also, dentin barrier test by millipore substitute showed constant results compared with the one by 500 μm thickness of bovine dentin disc.The cell
viability by 40-pcf polyurethane disc was not statistically different from that by bovine dentin disc (P > 0.05). Futurabond DC ® resulted in the highest cell viability and Bond force ® the lowest by the 40-pcf polyurethane disc (P < 0.05). The adhesives on the 40-pcf polyurethane disc changed cellular morphology with different degrees on the SEM images.In conclusion, filter combination of 0.65 μm + 0.45 μm + 0.22 μm and 40-pcf polyurethane disc can replace bovine dentin used in a traditional dentin barrier test and those can make more constant results in the test. Especially, considering the structural stability, 40-pcf polyurethane disc is recommended as more suitable substitute than a filter combination. Dentin barrier test by standardized substitutes can be a useful method for considering the potential toxicity of materials applied to dentin prior to their clinical adaptations and reducing the variations of bovine dentin discs that have different characteristics individually.
Files in This Item:
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Appears in Collections:
2. College of Dentistry (치과대학) > Others (기타) > 3. Dissertation
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/134933
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