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Bioengineering of vascular grafts using multilayer of PLCL nano-fibers with endothelial cells and fibroblast cells

Other Titles
 PLCL의 전기방사를 이용하여 개발한 지지체에 human umbilical vein endothelial cells과 human dermal fibroblast cells의 co-culture system을 이용한 소구경 인 
Authors
 이보람 
Issue Date
2014
Description
Dept. of Medical Science/석사
Abstract
Tissue engineering (TE) provides effective way to regenerate damaged or lost tissues by combining cells and biomaterial scaffolds to reconstruct and regenerate new tissue or organ. Diseases of vascular systems are the fatal causes of death and disability in people. Vascular grafts have been used successfully to replace large-diameter (diameter > 6mm) blood vessel, However the patency of small-diameter(diameter <6mm) vascular graft is still disappointing.We looking forward this study that we can make the successfully performed small diameter vascular graft using nano- fibrous scaffold as ECM construct in native vessels, and co-culture system of endothelial cells and fibroblast cells on the scaffolds provide proper micro-environment.For investigating the effect of scaffold with HDFs layer for cultured different term, we designed 2D co-culture experiment which is in-direct contact condition. In an indirect contact co-culture of fibroblasts and endothelial cells, it found that confluent HDFs layer influenced the HUVECs tube-like structures. In vitro static culture conditions, It was observed that the nano-fibrous scaffold with fibroblast layer supported HUVECs survival in the middle of vessel structure after 1, 7days in culture. However, the examination described that tubular structure did not maintain after 1month in static culture. Therefore we designed in vivo experiments which is the co-cultured vascular grafts were implanted in the nude mice for 2,4weeks. Histological analysis showed that co-culture system generated cellular infiltration into the multilayer of PLCL fibers and also collagen depositions were observed in the outer area of co-cultured vessel by post operated 4weeks.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 2. Thesis
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/134855
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