Guided bone regeneration using cyanoacrylate-combined calcium phosphate in a dehiscence defect : a histologic study in dogs

Abstract

Purpose: This study evaluated the effects of cyanoacrylate-combined calcium phosphate (CCP) as a candidate for a barrier membrane substitute in guided bone regeneration and the space maintenance capability of CCP placed in a dehiscence defect model.

Materials and Methods: Six standardized dehiscence defects (5 × 3 mm; height × width) around the dental implants were created on unilateral edentulous ridges in 5 dogs, where each defect was treated with sham surgery,biphasic calcium phosphate (BCP), CCP, barrier membrane (MEM), BCP+MEM, and CCP+MEM. The animals were sacrificed after an 8-week healing interval for histologic and histometric analyses.

Results: BCP and CCP sites showed increased bone formation compared to the control sites, although incomplete defect resolution occurred; bone regeneration heights (area) averaged 3.52±0.69mm (4.94±2.59mm2), 3.51±0.16mm (4.10±1.99mm2), and 1.53 ± 0.42mm (1.01±0.74mm2) for BCP, CCP, and control sites, respectively. All the MEM sites showed more bone formation compared with the sites that received the same biomaterials without a MEM, and BCP+MEM and CCP+MEM sites showed extensive bone formation within the defect and on the top of the implant; bone regeneration heights (area) averaged 3.96±2.86mm (12.46±11.61mm2), 5.45±0.25mm (11.63±1.97mm2), and 2.62 ± 0.27mm (3.43±0.98mm2) for BCP+MEM, CCP+MEM, and MEM sites, respectively.

Conclusions: CCP can be a good scaffold for supporting a MEM as opposed to acting as a substitued for the MEM in guided bone regeneration.