Effects of Epigallocatechin-3-gallate Coated Biphasic Calcium Phosphate on the Dehiscence Bony Defect around Dental Implant

Abstract

Purpose: Various kinds of the surface treatment have been tested in order to improve not only the osteoconductive effect but also the osteoinductive effect on BCP. Out of them, Recombinant human bone morphogenetic protein (rh-BMP) was known as a growth factor which shows excellent effects on bone regeneration. Out of various osteoinductive agents, green tea extract (epigallocatechin-3-gallate) has been recently in the limelight. From the previous studies, EGCG could be considered as a substance for enhancement of bone formation. Therefore, the purpose of this study was to evaluate osteogenic potential of BCP coated with low-concentrate ErhBMP-2 and EGCG.Materials & Methods: In this study, 5 mongrel dogs were used. Mandibular premolars and molars were extracted and bone defects (Ø 4 X 5 mm) were formed after 12 weeks healing period. Then, defect sites were treated with bone graft materials and membranes after implantation. In this experiment, BCP (OSTEONTM) was used as the control group. For the experimental groups, BCP coated with ErhBMP-2 0.05mg/ml and EGCG 5mg/ml+ ErhBMP-2 0.05mg/ml together was used. Dogs were euthanized at 6 weeks and 12 weeks after implantation. Results: The healing of the defect site and osseointegration of the implants was evaluated by histologic and histometric analyses.1.When comparing between the three groups, ErhBMP-2/EGCG group appeared to have higher value in bone to implant contact (BIC), bone density (BD), bone regeneration height (BRH) and bone mineral apposition rate (BMAR). But significant difference was not found. 2.BIC, BRH and BMAR values increased when comparing by time in the three groups. - BCP, ErhBMP-2 and ErhBMP-2/EGCG groups showed higher BIC value in the 12 week group than the 6 week group with significant differences.(p<0.05) - BCP group showed higher BRH value in 12 weeks with significant difference.(p<0.05) 3.In fluorescence analysis, bone remodeling around graft material and implant was more active in the ErhBMP-2/EGCG group.Conclusion: Within the limit of this study, the surface modification of BCP with low-concentrate BMP and EGCG was effective in enhancing the osteogenetic potential.