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Aluminum-fluoride complexes induce osteoclastogenesis

Other Titles
 알루미늄-불소 복합체의 파골세포 분화기전 연구 
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Dept. of Dental Science/박사
Receptor activator of NF-kB ligand (RANKL)-induced osteoclastogenesis is accompanied by Ca2+ signaling which sequentially activates calcineurin and NFATc1 and triggers osteoclastogenesis. However, it is not known whether RANKL-independent Ca2+ signaling could be induced and activates differentiation of osteoclasts during osteoclastogenesis. In the present study, the effect of aluminum-fluoride complexes, a G-protein activator, on Ca2+ signaling and osteoclastogenesis in mouse leukemic macrophage cell line, RAW 264.7 cells, and primary cultured mouse bone marrow derived macrophages was investigated. Using micro-CT and toluidine blue staining, decreased bone density was observed in the group of mice exposed to aluminum-fluoride complexes in drinking water for eight weeks. Aluminum-fluoride complexes induced the generation of extracellular Ca2+-dependent intracellular Ca2+concentration ([Ca2+]i) oscillations. Moreover, aluminum-fluoride complexes enhanced the generation of RANKL-mediated TRAP positive osteoclasts and induced the activated MAPK signaling such as ERK and JNK proteins. These results suggest that G-protein activation by aluminum-fluoride complexes could induce [Ca2+]i oscillations and osteoclast differentiation in osteoclast precursor cells.
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2. College of Dentistry (치과대학) > Others (기타) > 3. Dissertation
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