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MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line

Other Titles
 난소암 종양세포주 OVCAR3 에서 세포표면에 CD133을 발현하고 공모양을 형성하는 세포집단에서의 microRNA의 발현 양상 연구 
Issue Date
Dept. of Medicine/박사
Objectives: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line.Methods: Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with > 98% purity via cell sorting, miRNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile.Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and three of which were significantly down-regulated, including miR-1202 and miR-1181.Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.
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2. 학위논문 > 1. College of Medicine > 박사
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