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MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line
DC Field | Value | Language |
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dc.contributor.author | 남은지 | - |
dc.date.accessioned | 2015-12-24T08:40:45Z | - |
dc.date.available | 2015-12-24T08:40:45Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/133973 | - |
dc.description | Dept. of Medicine/박사 | - |
dc.description.abstract | Objectives: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line.Methods: Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with > 98% purity via cell sorting, miRNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile.Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and three of which were significantly down-regulated, including miR-1202 and miR-1181.Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line | - |
dc.title.alternative | 난소암 종양세포주 OVCAR3 에서 세포표면에 CD133을 발현하고 공모양을 형성하는 세포집단에서의 microRNA의 발현 양상 연구 | - |
dc.type | Thesis | - |
dc.contributor.department | Dept. of Obstetrics and Gynecology (산부인과학교실) | - |
dc.contributor.localId | A01262 | - |
dc.identifier.url | https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000113947 | - |
dc.contributor.alternativeName | Nam, Eun Ji | - |
dc.contributor.affiliatedAuthor | 남은지 | - |
dc.type.local | Dissertation | - |
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