Investigation of bio-active compounds inducing osteoinductive activity in Mesenchymal stem cell

Abstract

The current approach in biomaterial design of bone implants is to induce in situ regeneration of bone tissue and thus improve integration of the implants and reduce their failure. Therefore, new bioactive compounds which can stimulate migration, differentiation of osteoblasts and the following bone formation need to be studied. This study examines a group of low molecular weight compounds (both derived from natural products as well as chemically synthesized) on their osteoinductive abilities in human mesenchymal stem cells. Possible candidates for future use in bone tissue engineering are evaluated by examination of early markers of differentiation (such as ALP activity and collagen type I expression) as well as late markers of osteoblast differentiation (bone nodule formation). As bone remodeling and implant osteointegration depend not only on osteoblast response but also on interaction of the biomaterial with bone-resorbing osteoclasts, differentiation of osteoclasts in response to the bioactive compounds is also observed