Lithium ion이 家兎 血中 酒精濃度에 미치는 影響에 關한 實驗的 硏究

Abstract

[한글]

[영문]

Lithium, since it's discovery in 1818 by arfwedson, has been tried in the treatment of various diseases, including urinary calculi, gout and epilepsy. As the rationale of these treatment could not be established, most of these treatments are now considered useless or contraindicated. Since Cade, in 1949, made an astute observation of calming effects of lithium salts on guinea-pigs, renewed attention has been focused on the effects of lithium ion. In recent years it has been midely accepted that the lithium ion is an extremely promising and interiguing therapeutic

agent in the treatment of mania and other psychotic excitements, Chlororomazine has been used in the treatment of various excitements for upwards of a decade. It was reported that chlorpromazine increased the blood alcohol level in rabbits

significantly.

So far no work has been done on the effects of lithium ion on blood alcohol level. Therefore, to investigate this area, the author conducted animal experiments of the effects of lithium ion in combination with chlorormazine and reserpine on blood alcohol level in rabbits.

Material and Method

1. The exoerimantal work was done on mature rabbits of both sexes weighing between 2.0 and 3.0kg.

2. The experiment was done in 2 groups; control and experimental group.

3. Control group was devided into 3 subgroups; alcohol, chlorpromazine and reserpine subgroup. and experimental group was devided into 3 subgroups; lithium, lithium and chlorpromazine, and lithium and reserpine subgroup.

4. All intravenous injection was done into the marginal eavein of the rabbit. Lithium ohloride solation, 6.336%, was given in a dose of 3.0 mEq/kg of body weight daily for 4 days by intraenous route, and the last dose was given 1 hours before alcohol administration.

5. Chlorormazine, 5%, was given in a dose of 3.0 mg/kg of body weight one and half hours before alcohol administration by intravenous and intramusoular route respectively.

6. Reserpine was given in a dose of 3.0 mg/kg of body weight by intramuscular route at 30 minutes, 6, 12 and 24 hours before alcohol administration in control group, and at 6 and 12 hours before in experimental group.

7. In all groups, 20% ethanol solution was given in a dose of 5.0 ml/kg of body weight in 5 minutes exactly by intravnous route.

8. all of the blood specimens were obtained by cardiac puncture at 15 and 45 minutes after alcohol administration, and in alcohol control group those were obtained also at 30 and 60 minutes after alcohol administration.

9. The ethanol level was determined by Cavett's method.

Results

1. In lithium alone group, statistically it was significant that lithium increased the blood alcohol level in rabbits at 14, 30, 45 and 60 minutes after alcohol adinistration (P<0.01, P<0.01, P<0.02 and P<0.05 respectively).

2. In chlorpromazine alone group, when it was administered by intravenous route, chlorpromazine increased significantly the blood alcohol level in rabbits at 45 minutes after alcohol administration (P<0.05), and when it was administered by

intramuscular route at 15 minutes after alcohol administration, the alcohol level was significantly increased(P<0.01).

3. In lithium and chlorpromazine combined group, when chlorpromazine administered by intravenous route, lithium and chlorpromazine increased significantly the blood alcohol level at 15 minutes after alcohol administration (P<0.005), and when

chlorpromazine was administered by intramuscular route, the blood alcohol level was increased more significantly at 15 and 45 minutes after alcohol administration (P<0.001 respectively).

4. In reserpine along group, when it was administered 6 and 12 hours before alcohol administration, reserpine decreased significantly the blood alcohol level in rabbits at 15 and 45 minutes after alcohol administration (P<0.001 respectively).

5. In lithium and reseroine combined group, the blood alcohol level was increased more significantly at 15 and 45 minutes after alcohol administration (P<0.001 respectively) when reserpine was given 6 and 12 hours before alcohol administration.

Conclusions

1. The intravenous injection of lithium chloride alone in a dose of 3.0 mEq/kg increased significantly the blood alcohol level in rabbits.

2. The intravenous and intramuscular injection of chlorpromzine alone in a dose of 3.0mg/kg increased the blood alcohol lvel in rabbits significantly, and it was more significant when lithium was combined with chlorpromazine intramuscularly.

3. The intramusculr injection of reserpiae in a dose of 3.0 mg/kg decreased the blood alcohol level in rabbits significanbly, but when lithium was combined, the blood alcohol level was reverted to a significant elevation.