골종양(骨腫瘍)에 관한 전자현미경학적 연구

Abstract

[한글]

[영문]

The present study is concerned with the fine structure of the cells of bone tumors in patients who have been admitted to Severence hospital from Nov. 1, 1968 to Oct. 31, 169.

This is a preliminary study to investigate the cytochemical change in bone tumor cells.

Cowdry(1955) stated that cancer cells do not have any morphological feature visible in light microscope which could be exclusively demonstrated only in tumors. This also remains true for the electron microscopic findings. Dalton et al(1956),

Howatson et al(1957), in comparing the ultrastrurcture of the normal cell with a number of tumor cells reported that no single morphological trait is specific for the neoplastic cell.

Bernhard et al(1961) stated the difference in the cancer cell from normal cell is related to the tissue of origin. Various secretory products have been noted. The absence or the reduction in the size of the Golgi complex is one of characteristic feature for the highly differentiated tumor cell. Many vesicled and vacuoles containing dense granules or filaments have been described. Howatson et al(1955) described rat liver tumor cells noting an increase in the nucleo-cytoplasmic ratio, enlargement of the nucleoli, decrease in the number and size of mitochondria,

scant, poorly organized Ergastoplasmic membrane, and irregularity of the nucleus. Bernhard(1958) found plemorphism, enlargement of the endoplasmic reticulum, lobulation of the nucleus, decrease in number and size of mitochondria, and degeneration of the ground substance in tumor cells. Anderson(1963) described a bizarre nuclear and cellular configuration, a paucity of rough walled microsomal vesicles which were localized at the periphery of the cell, and large numbers of lipoid droplet in both the cell and matrix from a chondrosarcoma. Harada (1962), studying ostecgenic sarcoma cells found an increase in mitochondria andondoplasmic

reticulum, and lipoid granules connected with the mitochondria and an irregular, electron dense, fine inorganic structure in the cytoplasm in addition to the general characteristics of the tumor cell. Shipkey (1964) describing alveolar soft part sarcoma cells noted mmerous mitochondria with concentric cristae, extensive

well developed Golgi complex, sparse endoplasmic reticulum, intracellular collection of glycogen granules, and dense granules in the crystalline structure. Cells in the pressnt study came from Chondrosarcoma(1), Enchondroma(1), Alveolar soft part sarcoma(1), Synovial chondromatosis(1), Metastatic tumor(3), Epidermoid carcinoma(1), fibrous dysplasia(1), Reticulum cell sarcoma(1), and Osteogenic sarcoma(3). However electron microscopic observations could not be made from the following; Epidermoid carcinoma(1), Metastatic tunor(2), Enchondroma(1), Fibrous dysplasia(1) and Reticulum cell sarcoma(1), Immediately afer biopsy the specimen is cut into 1 cubicm. cubes and fixed in a mixture of 1% osmium tetroxide phosphate buffer for one and half hour and then cleansed twice with phosphate buffer(Ph, 7.4)

for 15 minutes ad dehydrated with graded othyl alcohol and propylene oxide and then put into a mixture of Epon 812 and propylene oxide in ratio of 1 and 1 for 1 or 4 hours and in ratio of 1 and 2 for 4 or 12 hours. Then the specimen is embedded in

Epon 812 and polymerized in the incubator at 35℃. 45℃. for 12 hours and 65℃. for 24 hours and sectioned in thickness of 400 or 600 Å with the Porter-Blum ultramicrotome MT-2, then the section is stained with uranyl acetate and lead citrate and observed with the electron microscope, Hitachi H U ll- E type.

The findings are summarized;

A. Chondrosarcoma

1. The nucleus is irregular, ovoid, and granular with deep notches, indentation and invagination. The nuclear membrane is distinct with many pores.

2. Glycogen particles and patchy accumulation of RNA granules are observed in the cytoplasm. The plasma membrane is distinct except at the tip of process. Endoplasmic reticulum and mitochondria and decreased, but the former are near the

cell membrane, the later are located perinuclearly. Rough walled vesicles with RNA granules and lipoid droplets near the cell membrane are observed in the cytoplasm. B. Osteogenic sarcoma

1. Irregularity of tumor cells, variation in cell communication, enlargement of the nucleus and nucleolus, and gathering of fine granules of the nucleus are apparent.

These findings may be considered as general characteristics of the tumor cells.

2. Contrary to the decrease of mitochondria and endoplasmic reticulum seen in the animal experimental tumor and the human cancer cells, these structures tend to increase in osteogenic sarcoma.

3. Irregular electron-dense fine inorganic structure are observed in the eytoplasm. C. Metastatic tumor

1. An irregular hyperchromatic enlarged nucleus with multiple notches, distinct membrane, and granular multiple enlarged nucleoli are observod.

2. Many large vacucles, enlarged distorted mitochondria, and decreased endoplasmic reticulum are observed in the cytoplasm.

D. Alveolar soft part sarcoma

1. One or two nuclei with prominent nucleoli are observed.

2. In the cytoplasm there are numorous irregular mitochondriae, an extcnsivc and well devcloped Golgi apparatus, and endoplasmic reticulum. An abundant endoplasmic reticulum in somo areas of the cytoplasm is called as Ergastoplasmic Nebenkern. The cristae of the mitochondria show prominent angulation on their outer asoect.

Peripherally they are arranged in a concentric fashion and enclose a granular central matrix.

3. The characteristic crystals in the cytoplams are rhmboid of variable size and have a single membrane and alternating light and dark line. E. Synovial chondromatosis

1. Adeep notch and indentation of the nucleus are observed with frequent pores.

2. In the cytoplasm an increased endoplasmic reticulum with distended cisternae is observed.

3. Many cytoplasmic foot-lets and pinocytic vesicles are see.

4. The intercellular space contains abundant collagenous fibrils.