표재성 진균의 thiocarbanilide(L-1) 처리 효과에 대한 전자현미경적 관찰

Abstract

[한글]

[영문]

Since the electron microscope was designed by Knoll and Ruska in 1931, it has been well applied in many scientific fields. especially in biological and medical fields. With the electron microscope, the ultrastructure of the cell was revealed and those of the bacteria and fungi were also known by many investigators.

In recant time, with electron microscope many investigators observed and reported that the cellular structures of the fungi, especially superficial fungi consists of nucleus, mitochondria, endoplasmic reticulum, secretory granules and inclusions.

And they emphasized the ultrastructure of the superficial fungus has similar structure of eucaryotic cells.

The cellular structure of superficial fungi and the exposure to the drugs also reported by many investigators(Adams, 1963; Gale, 1963; Lee et al, 1960; Blank et al., 1960).

A series of studies was made on cytological effects of L-1, one of the derivatives of thiocarbanilide, through the electron microscopic observation.

Materials and Methods

A. Materials

1. Chemical compound used

the synthetic chemical compounds of thiocarbanilide, namely L-q wre used in the experiment.

2. Strains of fungus used

Tr. rubrum, 1: Tr. mentagrophytes, 1: Epidermophyton floccosum, 1: and Microspoum canis, 1: strains wer used as experimental strains.

3. The media used

Sabouraud's glucose agar media containing 10r, 20r, 40r, 80r, 100r, 200r, 400r, 800r, and 1,000r per ml were prepared for the experiment.

B. Method

1. Evaluation of antifungal activity

the mechanism of the acquirement of resistance of superficial fungi to the antifungal agents is still obsoure. resistant patterns were evaluated through subinoculation into medium containing antifungal agent(L-1) serially diluted, and through a study of resistant patterns carefully.

2. Electron microscopic observation

The morphological changes were induced by treatment with thiocarbanilide(L-1).

For this examination, the colonies which had been clutured in the drug containing made a were centrifuged and fixed with 1% osmium tetroxide. After fixation and embedment in Epon 812, electron microscopic examinations were performed with HU-11

E-1 type of electron microscope manufactured by Hitachi Company.

A. Results of induced resistance

Each superficial fungi have a tendency to obtain a resistance temporarily within fixed duration. But by continuous exposure to the L-1, each superficial fungi appeared to have decreasing phenomenon of resistance.

B. Results of observation with electron microscope

1. In contrast to the bacteria, the normal fungus contains nuclear membrane, mitochondria, endoplasmic reticulum, demarked cell wall and secretory granules as observed in higher plants and animals.

2. Thickening of the cell wall, inapparent cell membrane with the appearance of electron thin area(ETA) and increase of inclusions were observed in the L-1 treated groups.

3. Thickening of cell wall increase of ETA were more apparent in the Epidermophyton floccosum than the other groups.

4. Increase of electron thin area(ETA) was thought to be associated with autolysis.