당뇨백서 악하선에서의 Na+, K+, activated adenosine triphosphatase에 관한 실험적 연구

Abstract

[한글]

Alloxan 투여로 오는 insulin 결핍증이 체중 및 하악선 중량변화에 미치는 영향을 관찰하고, insulin 투여시의 회복정도와 악하선내 Na**+ , K**+ ATPase의 활성을 측정하여 다음과 같은 결론을 얻었다.

1. Alloxan투여군은 대조군에 비하여 체중 및 하악선 중량이 각기 16%, 20% 감소되었으며 insulin 투여후 체중 및 악하선 중량은 모두 회복상을 보였다.

2. 각 실험군의 혈당은 대조군이 93.5±2.74㎎%, Alloxan 투여군이 230.3±16.05㎎%, insulin 투여군이 110.6±3.70㎎%이었다.

3. Na**+ , K**+ ATPase 활성은 deoxycholate로 두 번 처리한 결과가 한번 처리한 결과보다 9.3% 정도 높았다. 그러나 Mg**++ ATPase는 변화가 없었다.

4. Na**+ , K**+ ATPase 활성은 대조군에 배해 Alloxan투여군이 20% 감소되었으나 insulin 투여후 정상으로 회복되었다.

이상의 결과로 보아 Alloxan 유도 당뇨백서에서 약하선 중량의 감소와 Na**+ , K**+ ATPase의 활성감소가 타액선 분비에 밀접한 관계가 있는 것으로 사료된다.

[영문]

Effect of insulin deficiency and insulin replacement therapy on body weight, the weight of submaxillary gland and Na**+ , K**+ adenosine triphosphatase(Na**+ , K**+ ATPase) in submaxillary gland of albino rats were observed. Experimental study of

the Na**+ , K**+ ATPase of submaxillary gland was done by a doxycholate-treated procedure and the results were obtained as follow;

1. Alloxan treated group revealed marked decrease of the body weight and weight of submaxillary gland than control group. These changes were recovered by insulin replacement therapy.

2. Blood glucose level of control group was 93.5±2.74mg%, alloxan group was 230.3±16.05mg%, and insulin treated group was 110.6±3.7mg% respectively.

3. Result of double treatment of sodium deoxycholate on the tissue of submaxillary gland was presented higher(9.3%) specific activity of Na**+ , K**+ ATPase than single treatment of sodium deoxycholate, but specific activity of Mg**++ ATPase was not influenced by the times of treatment of sodium deoxycholate.

4. The specific activity of Na**+ , K**+ ATPase of alloxanized rats was lowered about 20% than control group. These change were recovered by insulin replacement therapy.

There results suggested that the lower specific activity of Na**+ , K**+ ATPase and loss of weight of submaxillary gland had close effect on salivary secretion.