항암 화학요법제가 Hamster 협낭점막의 DMBA 유도 구강암에 미치는 영향에 관한 실험적 연구

Abstract

[한글]

저자는 golden Syrian hamster 92마리를 실험군 66마리, 대조군 26마리로 나누어 실험군은 heavy mineral oil에 용해한 0.5% DMBA를, 대조군은 생리식염수(위약)로 실험동물의 양측협낭점막에 격일간격으로 12주간 도포하여 실험종양을 발생시킨후 실험군 36마리와 대조군 24마리는 각각 18마리와 12마리씩 나누어 Ⅰ,Ⅱ군으로 구분하여 실험군 및 대조군의 Ⅰ군은 Bleomycin을 단일투여했으며, Ⅱ군은 Bleomycin과 cis-Platinum을 병용투여하

여 각군별로 1, 3, 7, 14, 21, 28일째 희생시켜 암조직 및 점막조직을 적출하여 각각 통법에 따라 광학현미경 및 전자현미경표본을 제작하여 염색한 후 육안적, 광학현미경적 및 전자현미경적으로 변화를 비교관찰하여 다음과 같은 결론을 얻었다.

1. 육안적으로 1차실험의 실험군에서 암의 발생율은 7주째 45%, 9주째 75%, 12주째 100%이며 실험중 사망률은 30%이다.

2. 1차실험의 실험군에서 6주에 상피의 증식율, 8주에 유두종을, 13주에 암세포의 경미한 침윤을 15주에 침윤성편평상피암이 관찰되었다.

3. 2차실험의 실험군 Ⅰ군에서 3주부터, Ⅱ군에서 2주부터 다형성이 감소되는 소견을 보였다.

4. 혈관의 확장상은 2차실험의 실험군 Ⅰ군에서 3주째, Ⅱ군에서 2주째 가장 심했으며 Ⅰ군보다 Ⅱ군에서 더욱 심했다.

5. 2차실험의 실험군에서 1일째에만 공포변성이 관찰되었으며 염증 및 괴사상은 Ⅰ,Ⅱ군에서 공히 관찰되었고 부종은 Ⅰ군에서 3주째에, Ⅱ군에서 2주째에 현저하였다.

6. 전자현미경적으로 공포변성은 2차실험군에서 공히 초기에 관찰되었으며 기저막의 파괴상이 실험군 전체에서 관찰되었다.

7. 2차실험 실험군에서 Ⅰ군에서 3일째 위족(pseudopodia)이 현저하였으며, 실험군 대조군 공히 미토콘드리아의 파괴상을 보였으며 실험군 Ⅱ군보다 Ⅰ군에서 더 심했다.

[영문]

This study was undertaken to observe the DMBA- induced buccal pouch carcinoma in hamster and the effects of single dose of antineoplastic agents : Bleomycin and cis-Platinum.

In this study, ninety two golden Syrian hamsters, weighing about 100 grams were used.

The first experiment was carried out to produce experimentally induced - buccal pouch carcinoma by the application of 0.5% DMBA in heavy mineral oil on buccal pouch mucosa of hamsters in every other day for 12 weeks.

Experimental animals were divided into two groups, sixty six experimental group treate with 0.5% DMBA in heavy mineral oil and twenty six control group treated with saline solution as placebo.

The animals of experimental group were sacrificed serially on the 6th, 7th, 8th, 9th, 10th, 11th, 12th, 13th, 14th, 15th. weeks after application of carcinogen.

The second experiment was done by the administration of antineoplastic agents, Bleomucin and cis-Platinum in the hamster of DMBA induced buccal pouch carcinoma.

36 animals of experimental group and 24 animals of control group were diveded into two groups(Ⅰ,Ⅱ).

Group Ⅰ of experimental and control group were administrated Bleomycin

Group Ⅱ experimental and control group were administrated Bleomycin and cis-Platinum.

All animals were sacrificed serially on the 1st, 3rd, 7th, 14th, 21st, 28th day after intra peritoneal injection of antineoplastic agents.

At sacrifice, tumor masses were excised and examined grossly, microscopically and electromicroscopically.

After examination and comparison of all specimens, following results were obtained;

1. Grossly, in the first experiment, papillomas were developed at 45% in the 7th week, 75% in the 9th week, 100% in the 12th week respectively, and mortality was about 30% during the experiment.

2. Microscopically, in the first experiment, epithelial hyperplasia in the 6th week, papilloma in the 8th week, microinvasion in the 13th week and infiltrative squamous cell carcinoma in the 15th week were observed respectively.

3. In the second experiment, decreasing tendency of pleomorphism was observed from the 21st day in group Ⅰ, the 14th day in group Ⅱ.

4. Microscopically, in the second experiment, marked vascular dilatation was observed in the 21st day of group Ⅰ, the 14th day of group Ⅱ and that was prominent in group Ⅱ than that of group Ⅰ.

5. In the second experiment, vacuolar degeneration was observed in the 1st day of group Ⅰ, Ⅱ of experimental group and inflammation and necrosis were observed in Ⅰ, Ⅱ experimental groups.

Edema was observed in the 21st day of Ⅰ group, in the 14th day of Ⅱ experimental group.

6. Electronmicroscopically, vacuolation was observed in Ⅰ, Ⅱ experimental groups in the second experiment and discontinuities of basement membrane was also observed in all experimental groups.

7. In the second experiment, pseudopodia was prominent in the 3rd day of Ⅰ experimental group and mitochondrial changes were observed in all experimental and control groups and that was prominent in group Ⅰ than that of group Ⅱ.