Phenobarbital 투여가 간장내 알콜대사에 미치는 영향

Abstract

[한글]

저자는 체중 200gm 내외의 웅성백서에 phenobarbital을 체중 kg당 100mg를 stomack tube를 사용하여 매일 1회씩 1주일간 투여한 후 체중변화 및 간장내 에타놀대사에 관여하는 효소들의 변화를 측정 관찰하였든 바 다음과 같은 의의있는 실험결과를 얻었다.

Phenobarbital 투여가 체중에는 변화를 가져오지 않았으나 대조군에 비하여 간장무게는 53.9%, microsomal protein함량은 55% 증가하였다.

간장세포내 cytosol의 alcohol dehydrogenase(ADH) 활성치는 단백질 mg당 및 간장조직 gm당 표시하면 대조군과 하등의 차이를 보이지 않았으나 총간장내 ADH활성치는 phenobarbital투여로 대조군에 비하여 48%가 증가하였다. 이는 ADH활성 또는 함량 증가에 의한 것이 아니라 전체 간장조직의 증대에 따른 결과로 사료된다. 이와는 달리 간장 microsome내외 MEOS활성은 단백질 mg당에서는 대조군에 비하여 119%가 증가하고 간장조직 gm당에서는 171.5%로 의의있게 증가하였으며, 총간장내 MEOS활성치는 319%나 증가하였다. Microsomal NADPH ??idase활성치는 mg당 46.8%, gm당 128.8% 증가하였고 총간장내 NADPH oxidase는 252% 증가하였다.

이상과 같이 phenobarbital 투여는 간장조직 비대 및 microsomal protein 함량을 증가시키며 에타놀대사에 관여하는 효소들의 함량증가로 에타놀대사가 증가되며, 특히 phenobarbital 투여로 에타놀산화 증가는 MEOS활성 및 MADPH oxidase 활성증가보다는 간장조직 비대에 의한 ADH의 양적증가가 더욱 중요한 요인으로 사료된다.

[영문]

The administration of phenobarbital (100mg/kg) ti rats bt stomach tube daily for one week resulted in significant increases in liver weight and microsomal protein concentration by 53.9 and 55 per cent respectively over that of the control group.

No significant increase in cytomplasmic alcohol dehydrogenase activity was observed, when it was expressed per mg of protein and gm of fresh liver; however, the total activity of cytoplasmic alcobol dehydrogenase in the whole liver increased signifficantly by 48 per cent. This was due to the fact the total weight of liver was imm?????in phenobarbital treamed rats.

The activity of the hepatic an??????? ??????? ?????? system (MEOS) increased by 119 per cent over that of the controls when it was expreased per mg of protein and increased by 171.5 per cent when it was expressed per gm of fresh liver weight. The

activity of hepatic microsomal NADPH oxidase also increased by 46 per cent over that of the control group when it was expressed in mg of protein and increased by 128.8 per cent when it was expressed in gm of fresh liver weight, indicating that the amount of these two enzymes was increased per gm of fresh liver when treated by phenobarbital.

The per cent of total ethanol oxidation by cytoplasmic alcohol dehydrogenase and microsomal oxidation system was 88 and 12 per cent respect tively in the control group; however, it was 72.5 and 27.5 per cent respecively in the phenobarbital treated group.

In spite of microsomal ethanol oxidation and microsomal proteins were increased significanty but cytoplasmic alcohol dehydrogenase activity in liver was not altered as such by phenobarbital treatment, accordingto our experimental results.

These results suggest that ethanol oxidation by cytoplasmic alcobol dehydrogenase in cytosol plays the main part and the microsomal ethanol oxidation system plays the minor part in the metaboliam of ethanol.