가토에 이식된 Titanium plasma sprayed IMZ 임프란트와 골의 계면 접촉 양상에 따른 결합력에 관한 연구

Abstract

[한글]

본 실험은 원주형의 titanium Plasma spray 처리 된 치과용 titanium 임프란트(IMZ)를 IMZ회사에서 추천하는 이식방법을 대조군으로, 임프란트보다 큰 골결손 부위 형성 후 이식방법을 실험 Ⅰ군으로, 임프란트 이식 후 골결손 부위에 다공성 hydroxyapatite 입자(I

nterpore 200** )를 동시에 이식하는 방법을 실험 Ⅱ군등 세군으로 나누어 가토의 대퇴골에 이식하여 8주 후 동물을 회생시켜 임프란트와 임프란트주위 조직사이의 전단강도를 pushout test로 측정 비교하였고, 또한 각군을 조직학적으로 관찰하여 다음과 같은 결과를

얻었다.

1. Titanium 임프란트와 주위조직 간의 전단강도는 대조군에서는 2.614±0.680 MPa, 실험 Ⅰ군에서는 0.664±0.322 MPa, 실험 Ⅱ군 2.281±0.606 MPa이었으며, 대조군과 실험 Ⅰ군, 실험 Ⅰ군과 실험 Ⅱ군은 통계학적 유의한 차이가 있었으며, 대조군과 실험 Ⅱ군은

유의한 차이가 없었다.

2. 3개군의 titanium 임프란트 주위의 골형성은 수질골보다 피질골에서 더 진행되었다.

3. 대조군과 실험 Ⅱ군의 비탈희 표본의 조직학적 소견은 계면의 50% 이상에서 골과 유골 형성이 관찰되었다.

4. 실험 Ⅰ군 비탈희 표본의 조직학적 소견은 계면의 50% 이하에서 골과 유골 형성이 관찰되었으며, 상부에서 부분적인 골결손이 관찰되었다.

5. 실험 Ⅱ군에서 다공성 HA입자 주위에 직접적인 골접촉 양상을 보였고, 부분적으로 거대 세포침윤이 관찰되었다.

6. Titanium 임프란트 및 다공성 HA입자 주위에서 염증세포는 관찰되지 않았다.

Titanium 임프란트 주위에 골결손부를 임의로 형성하여 다공성 HA입자를 이식한 경우 임프란트만 이식한 경우에 비하여 골형성이 보다 많았으며, 임프란트와 골의 결합력이 컸다.

A STUDY ON SHEAR BOND STRENGTH OF INTERFACE BETWEEN BONE AND TITANIUM PLASMA

SPRAYED IMZ IMPLANT IN RABBITS

Chong - Hyun Han

Dept. of Dental Science, Graduate School, Yonsei University

(Directed by Dong-Hoo Han, D.D.S., M.S.D., Ph.D.)

In recent years immediate implantation has been tried by a few clinicians. This

study placed IMZ implants in the rabbit femur with and without bony defects around

the implant for simulating fresh extraction site. And one group with bony defects

used porous hydroxyapatite granules(HA) to fill in, and the other group left the

bony defects around the implant.

The purpose of this study was to compare the shear bond strength and the bony

contact and formation around the implant.

Fifteen rabbits were divided into three groups and placed 10 IMZ implants to each

group. Implant sites were surgically prepared with IMZ drills kit and implants were

placed(Control), artificial bony defect was created with Apaceram drills kit around

the implant sites and implants were placed(Experimental Ⅰ), bony defect was filled

with porous hydroxyapatite granules(Experimental Ⅱ). Thereafter, rabbits were

sacrificed at 8th week and specimens were prepared and pushout tested for shear

band strength of bone - implant interface immediately. Undecalcified and

decalcified specimens were prepared with Vilanueva and hematoxylin - eosin stain

for light microscopic finding. The results of this study were as follows.

1. In the control group, mean shear strength of bone - implant interface was

2.614±0.680 MPa, experimental Ⅰ was 0.664±0.322 MPa, and experimental Ⅱ was

2.281±0.606 MPa. There was significant difference between control and experimental

Ⅰ, between experimental Ⅰ and experimental Ⅱ, but did not show significant

difference between control and experimental Ⅱ statistically.

2. In the bony formation surrounding IMZ implant of the three groups, that of

cortical bone is more advanced than cancellous bone area

3. In the histological findings of undecalcifed specimens, control and

experimental Ⅱ showed more than 50% of bony or osteoid formation at the bony -

implant interface.

4. In the histological findings of undecalcified specimens, experimental Ⅰ

showed less than 50% of bony or osteoid formation at the interface, and observed

partial bony defect in the coronal zone.

5. In the experimental Ⅱ group, were observed direct bony contact to

hydroxyapatite granules, and infiltration of a few giant cells.

6. No imflammatory responses were seen around the titanium implants and the

hydroxyapatite granules.

[영문]

In recent years immediate implantation has been tried by a few clinicians. This study placed IMZ implants in the rabbit femur with and without bony defects around the implant for simulating fresh extraction site. And one group with bony defects used porous hydroxyapatite granules(HA) to fill in, and the other group left the bony defects around the implant.

The purpose of this study was to compare the shear bond strength and the bony contact and formation around the implant.

Fifteen rabbits were divided into three groups and placed 10 IMZ implants to each group. Implant sites were surgically prepared with IMZ drills kit and implants were placed(Control), artificial bony defect was created with Apaceram drills kit around the implant sites and implants were placed(Experimental Ⅰ), bony defect was filled with porous hydroxyapatite granules(Experimental Ⅱ). Thereafter, rabbits were

sacrificed at 8th week and specimens were prepared and pushout tested for shear band strength of bone - implant interface immediately. Undecalcified and decalcified specimens were prepared with Vilanueva and hematoxylin - eosin stain

for light microscopic finding. The results of this study were as follows.

1. In the control group, mean shear strength of bone - implant interface was 2.614±0.680 MPa, experimental Ⅰ was 0.664±0.322 MPa, and experimental Ⅱ was 2.281±0.606 MPa. There was significant difference between control and experimental

Ⅰ, between experimental Ⅰ and experimental Ⅱ, but did not show significant difference between control and experimental Ⅱ statistically.

2. In the bony formation surrounding IMZ implant of the three groups, that of cortical bone is more advanced than cancellous bone area

3. In the histological findings of undecalcifed specimens, control and experimental Ⅱ showed more than 50% of bony or osteoid formation at the bony - implant interface.

4. In the histological findings of undecalcified specimens, experimental Ⅰ showed less than 50% of bony or osteoid formation at the interface, and observed partial bony defect in the coronal zone.

5. In the experimental Ⅱ group, were observed direct bony contact to hydroxyapatite granules, and infiltration of a few giant cells.

6. No imflammatory responses were seen around the titanium implants and the hydroxyapatite granules.