이하선 타액내 Parotid Isoelectric Focusing Variant Protein (PIF)의 유전적 다형현상에 관한 연구

Abstract

[한글]

이하선 타액 단백질에 관한 체계적인 연구의 일환으로 parotid isoelectric focusing variant protein (PIF)의 다형현상을 알아보기 위하여 임의로 선택한 20세부터 29세까지의 건강한 한국인 성인 남녀 150명을 대상으로 각각 이하선 타액을 채취하였다. 채취한 이하선 타액을 urea-polyacrylamide slab gel(pH 3.5-5.2)상에서 5시간 동안 isoelectric focusing한 후, Coomasie Brilliant Blue R-250 / 20% trichloroacetic acid (TCA)로 염색한 뒤 20% TCA 용액으로 탈색하여 한국인 집단을 대상으로 한 PIF 의 표현형 빈도와 각 유전자 빈도를 구하였고, 이 집단을 토착성씨군과 도래성씨군으로 나누어 각 군의 PIF 표현형 분포와 유전자 빈도를 구하여 다음과 같은 결과를 얻었다.

1. 한국인 이하선 타액내 parotid isoelectric focusing variant protein(PIF)의 표현형 분포는 PIF(+)형이 140명 (93.3%), PIF(-)형이 10명(6.7%)인 것으로 나타났다.

2. 한국인 이하선 타액내 parotid isoelectric focusing variant protein(PIF)의 유전자 빈도는 PIF**+ 0.742, PIF**- 0.258 인 것으로 나타났다.

3. 한국인 150명중 토착성씨군의 PIF 유전자 빈도는 PIF**+ 0.733, PIF**- O,267, 도래성씨군의 PIF 유전자 빈도는 PIF**+ 0,796, PIF**- 0.234 인 것으로 나타났다.

4. 한국인 집단 전체의 PIF 유전자 빈도는 도래성씨군의 PIF 유전자 빈도보다 토착성씨군의 유전자 빈도에 더 가까운 것으로 나타났다.

Genetic Polymorphism of Parotid Isoelectric Focusing Variant Protein(PIF) in a

Korean Population

Dong-Ho Han D.D.S., M.S.D

Department of Oral Diagnosis, The Graduate School, Yonsei University

(Directed by: Prof Chong-Youl Kim D.D.S., M.S.D., Ph.D.)

In order to identify the polymorphism of the parotid isoelectric focusing variant

protein(PIF), as an integrating part of systemic study about parotid salivary

proteins, this study was conducted. One-hundred and fifty healthy adult Koreans

between the ages of 20 and 29 were chosen, and their parotid saliva was collected.

The parotid saliva went under isoelectric focusing on a urea-polyacrylamide slab

gel(pH 3.5-5.2) for 5 hours. Then, it was stained with Coomasie Brilliant Blue

R-250/20% Trichloroacetic acid(TCA) and again was destained with 20% TCA solution.

The Phenotype frequency and gene frequency of each isoelectric focusing variant

protein(PIF) were obtained, and the subjects were divied into the indigenous family

name group and immigrated family name group. The phenotype and gene frequency of

each group were compared, and the following results were obtained:

1 . The phenotypes of parotid isoelectric focusing variant protein(PIF) observed

in parotid saliva of the 150 Koreans were PIF(+) in 140 people (93.3%) and PIF(-)

in 10 people (6.7%).

2. The gene frequency of PIF**+ was 0.742, and that of PIF**- was 0.258.

3. Of the 150 Koreans, the gene frequencies of the indigenous family name group

were 0.733 in PIF**+ and 0.267 in PIF**- ; those of the immigrated family name grou

p

were 0.796 and 0.234.

4. The gene frequency of the entire Korean group was closer to that of indigenous

family name group than the immigrated family name group.

[영문]

In order to identify the polymorphism of the parotid isoelectric focusing variant protein(PIF), as an integrating part of systemic study about parotid salivary proteins, this study was conducted. One-hundred and fifty healthy adult Koreans between the ages of 20 and 29 were chosen, and their parotid saliva was collected.

The parotid saliva went under isoelectric focusing on a urea-polyacrylamide slab gel(pH 3.5-5.2) for 5 hours. Then, it was stained with Coomasie Brilliant Blue R-250/20% Trichloroacetic acid(TCA) and again was destained with 20% TCA solution.

The Phenotype frequency and gene frequency of each isoelectric focusing variant protein(PIF) were obtained, and the subjects were divied into the indigenous family name group and immigrated family name group. The phenotype and gene frequency of each group were compared, and the following results were obtained:

1 . The phenotypes of parotid isoelectric focusing variant protein(PIF) observed in parotid saliva of the 150 Koreans were PIF(+) in 140 people (93.3%) and PIF(-) in 10 people (6.7%).

2. The gene frequency of PIF**+ was 0.742, and that of PIF**- was 0.258.

3. Of the 150 Koreans, the gene frequencies of the indigenous family name group were 0.733 in PIF**+ and 0.267 in PIF**- ; those of the immigrated family name group were 0.796 and 0.234.

4. The gene frequency of the entire Korean group was closer to that of indigenous family name group than the immigrated family name group.