Progesterone투여백서 치은 상피의 투과성에 대한 실험적 연구

Abstract

[한글]

저자는 사춘기, 월경시, 임신시에 치은염증이 급격히 증가하는 원인을 규명하기 위해 추적자로서 horseradiah peroxidase를 사용하여 progesterone이 치은상피의 투과성에 미치는 영향을 연구하였다.

실험동물로는 체중 200gm 내외의 자성 백서(Spraque Dawley계)40마리를 사용하여 10마리(대조군 2마리, 실험군 8마리)씩 4군으로 나누고, 백서당 progesterone 0.2㎖(progeste

rone 5㎎/㎖)을 1일 1회씩 근육주사하였다.

실험 1일, 5일, 10일, 15일째에 horseradish peroxidase를 15분간 협측 변연치은에 국소도포하고 즉시 희생시켰다.

희생시킨 즉시 하악골을 적출하여 formaldehyde-g1utaraldehyde고정액에 고정시키고, 0.5% 3.3 diaminobenzidine tetrahydrochloride용액에 배양시킨 후. 10% trichloroacetica

c acid에 탈회시킨 다음 동결절편하여 조직화학적으로 관찰한 결과 다음과 같은 의의있는 결론을얻었다.

1. Horseradish peroxidase는 각화가 되어있는 치은상피는 통과하지 못했으나 각화층이 없는 부착상피는 통과하였다.

2. 부착상피를 통해서 침투된 horseradish peroxidase정도는 대조군과 실험군사이에 차이가 있었다.

3. 실험군에서 progesterone의 투여기간이 길수록 horseradlah peroxidase의 침투 정도는 점진적으로 증가하였다.

AN EXPERIMENTAL STUDY ON THE PERMEABILITY OF RAT GINGIVAL

EPITHELIUM TREATED WITH PROGESTERONE

Jung Kiu Chai

Department of Dental Science The Graduate School, Yonsei University

(Directed by Prof. Yong Kwan, D.D.S., D.M.D)

This study was undertaken to observe the effect of progesterone to permeability

of gingival epithelium and horseradish peroxidase was used as a tracer.

Forty female rats were used in this experiment and divided into four groups. Each

group consists of 10 animals.

Each rat was injected intramuscularly with progesterone 0.2㎖(progesterone

5㎎/㎖)in experimental group.

On the first, fifth, tenth, fifteenth experimental day after injection,

horseradish peroxidase was applied to the gingival margin for 15minutes. At the end

of application animals were killed by cervical dislocation. The mandible with 1st

and 2nd molar portion was removed and immediately fixed in

formaldhyde-glutaraldehyde fixative. The mandible was incubated in 0.5% 3.3

diaminobenxidine tetrahydrochloride and then decalcified in 10% trichloroacetic

acid.

Decalcified tissues were frozened to section and observed histochemically.

The following results were obtained:

1. Horseradish peroxidase failed to penetrate through keratinized gingival

epithelium, but horseradish peroxidase penetrated through junctional epithelium.

2. The penetration of horseradish peroxidase through junctional epithelium was

different between control and experimental group.

3. In the experimental group, when the injection period of progesterone was

increased, the penetration of horseradish peroxidase was increased.

[영문]

This study was undertaken to observe the effect of progesterone to permeability of gingival epithelium and horseradish peroxidase was used as a tracer.

Forty female rats were used in this experiment and divided into four groups. Each group consists of 10 animals.

Each rat was injected intramuscularly with progesterone 0.2㎖(progesterone 5㎎/㎖)in experimental group.

On the first, fifth, tenth, fifteenth experimental day after injection, horseradish peroxidase was applied to the gingival margin for 15minutes. At the end of application animals were killed by cervical dislocation. The mandible with 1st

and 2nd molar portion was removed and immediately fixed in

formaldhyde-glutaraldehyde fixative. The mandible was incubated in 0.5% 3.3 diaminobenxidine tetrahydrochloride and then decalcified in 10% trichloroacetic acid.

Decalcified tissues were frozened to section and observed histochemically.

The following results were obtained:

1. Horseradish peroxidase failed to penetrate through keratinized gingival epithelium, but horseradish peroxidase penetrated through junctional epithelium.

2. The penetration of horseradish peroxidase through junctional epithelium was different between control and experimental group.

3. In the experimental group, when the injection period of progesterone was increased, the penetration of horseradish peroxidase was increased.