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수종의 항생제가 인공 stearic acid 단분자막에 미치는 영향

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Lipid단분자막은 생체막의 가장 간단한 모형으로 알려져 있다. 즉, 단분자막을 형성하고 있는 지방의 분자배열상태는 생체막의 지방배열과 유사하다. 따라서 많은 연구자들이 lipid단분자막을 사용하여 생체막에서 일어나는 약물작용을 간접적으로 추측한 바 있다.(Langmuir 1937, Shane과 Gershfeld 1960)

본 실험은 수종의 항생제 Kanamycin, streptomycin, amphotericin B, polymyxin B가 세포막 지질층에 영향을 미칠 것으로 추측하여 Langmuir-Adam표면압계를 수정제작한 automatic film balance를 사용하여 stearic acid 단분자막의 면적-장력 곡선을 측정했다.

kanamycin은 stearic acid단분자막을 응축시켰는데 이러한 작용은 Ca**++ 의 작용과 유사하였다.

streptomycin, polymyxin B 및 amphotericin B 등은 stearic acid단분자막의 표면압력을 증가시켰다.


It has been known that lipid monolayers represent the simples type of membrane model having an organized structure. The orientation of lipids in a monolayer is similar to that proposed for cell membranes. Therefore, the lipid monolayer has been used by a number of investigators(Langmuir 1937, shane and Gershfeld 1960) as a model system to study rechanisms of drug action in the biological membrane.

Recently, it has been reported that kanamycin reduces osmotic and mechanical fragilities of human red blood cells(Hong 1973) and streptomycin destructs the permeability barrier, releasing cell contents, such as nucleotide and amino acids Anand and Davis 1961).

We therefore investigated, in the present study, effects of several antibiotics(kanamycin, streptomycin, amphotericin B, polymyxin B) on stearic acid monolayer using a Langmuir trough, and obtained following results:

1) Kanamycin appeared to condense the stearic acid monolayer, as observed with ca**++ .

2) On the other hand, streptomycin, amphotericin B and polymyxin B expanded stearic acid monolayer, indicating that the surface pressure is increased.
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