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정상동물의 심근내 Catecholamines 분포에 관한 연구

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 Studies on the intracellular distribution of myocardial catecholamines of normal animals 
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[영문] Accumulated evidence indicates that the catecholamines are stored as granule within the celles of adrenal medulla or sympathetic nervous tissues. Myocardial catecholmines have been shown to be present in the particulate fraction as well as in the soluble fraction obtained by differential centrifugation of cell free suspension from homogenates of cardiac muscle (Potter and Axelrod, 1962; Wegman and Kako, 1962; Campos Shideman, 1962). Howere, according to these investigatore the ratio to that in the soluble fraction of myocardium varies considerably. This may be attributed to a species and fraction procedure difference. Present experiment was designed to determine the intracellular distribution of myocardial catecholamines, with special reference to the animal species. The heart was rapidly removed from animals under ether anesthesia and 5 volumes of 0.38 M ice-cold sucose in a waring blender. The resultant suspension was passed through a double layer of gauze to remove the fibrous tissue. The homogenate was then centrifuged at 3-5℃ for 10 minutes at 600 xg (low-speed centrifugation), which brought down incompletely fragmented cells and nucli. A low-speed supernatant was thus obtained, and was again centrifuged at 3-5℃ for 30 minutes at 15,000 xg (high-speed centrifugation), which fractionated into supernatant and sediment. The concentration of catecholamines in each fraction obtained by the above differential centrifugation, was determined spectrophotofluormetrically by the modified procedure of the method described by Shore and Olin (1958). 1. Examination of the intracellular distribution of the myocardial catecholamines of normal rabbits showed that a higher concentration of the aminos was present in the high-speed supernatant fraction (0.40ug/gm) and the ratio of supernatant/sediment was 4. The catecholamines present in the low-speed supernatant was almost completely recovered in both fractions obtained by high-speed centrifugation. 2. In the cardiac homogenate of normal cats, a similar intracellular distribution of catecholamines was observed. Thus, the concentrations of amines in high-speed supernatant and sediment were 0.48 and 0.09ug/gm respectively. 3.In the experiment with cardiac homogenate of normal rate, it was also found that higher concentration of catecholamines was present in the high-speed supernatant and the ratio of supernatant/sediment was 4.3 which was not significantly different from those observed in the cardiac homogenates of normal rabbits or cats. The concentrations of catecholamines in the high-speed supernatant and sediment fractions would not represent the actual distribution in vivo. However, these values are reproducible and relatively constant under the described experimental conditions. Therefore, these may be considered useful as a criteria for the study of intracellular distribution of myocardial catecholamines. From the above results, it may be concluded that there is no significant difference in the intracellular distribution of myocardial catecholamines of normal rabbits, cats and rats.
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2. Thesis / Dissertation (학위논문) > 1. College of Medicine (의과대학) > Master's Degree (석사)
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