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Dendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin/CD209 Is Abundant on Macrophages in the Normal Human Lymph Node and Is Not Required for Dendritic Cell Stimulation of the Mixed Leukocyte Reaction

 Angela Granelli-Piperno  ;  Alla Pritsker  ;  Ralph M. Steinman  ;  Thomas M. Moran  ;  Vincent Piguet  ;  Christine Trumpfheller  ;  Chae Gyu Park  ;  Jean-Francois Arrighi  ;  Irina Shimeliovich  ;  Maggi Pack 
 JOURNAL OF IMMUNOLOGY, Vol.175(7) : 4265-4273, 2005 
Journal Title
Issue Date
Animals ; Antibodies, Monoclonal ; Cell Adhesion Molecules/antagonists & inhibitors ; Cell Adhesion Molecules/genetics ; Cell Adhesion Molecules/immunology ; Cell Adhesion Molecules/metabolism* ; Cell Line ; Clone Cells ; Dendritic Cells/immunology* ; Dendritic Cells/virology ; Dogs ; HIV-1/immunology ; Humans ; Lectins, C-Type/antagonists & inhibitors ; Lectins, C-Type/genetics ; Lectins, C-Type/immunology ; Lectins, C-Type/metabolism* ; Lymph Nodes/cytology ; Lymph Nodes/metabolism* ; Lymphocyte Culture Test, Mixed ; Macrophages/metabolism* ; Mice ; Mice, Inbred BALB C ; RNA, Small Interfering/metabolism ; Receptors, Cell Surface/antagonists & inhibitors ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/immunology ; Receptors, Cell Surface/metabolism* ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Recombinant Proteins/metabolism ; T-Lymphocytes/virology ; Transfection
The C-type lectin dendritic cell-specific ICAM 3-grabbing nonintegrin (DC-SIGN)/CD209 efficiently binds several pathogens, including HIV-1. DC-SIGN is expressed on monocyte-derived DCs in culture, and importantly, it is able to sequester HIV-1 within cells and facilitate transmission of virus to CD4+ T cells. To investigate DC-SIGN function, we have generated new mAbs. We report in this study that these and prior anti-DC-SIGN mAbs primarily label macrophages in the medullary sinuses of noninflamed human lymph node. In contrast, expression is not detected on most DCs in the T cell area, except for occasional cells. We also noted that IL-4 alone can induce expression of DC-SIGN in CD14+ monocytes and circulating blood DCs. However, blockade of DC-SIGN with Abs and DC-SIGN small interfering RNA did not result in a major reduction in the capacity of these DCs to transfer HIV to T cells, confirming significant DC-SIGN-independent mechanisms. The blocking approaches did reduce HIV-1 transmission by DC-SIGN-transfected cells by >90%. DC-SIGN blockade also did not reduce the ability of DCs to stimulate T cell proliferation in the MLR. These results indicate that DC-SIGN has the potential to contribute to macrophage function in normal human lymph node, and that DCs do not require DC-SIGN to transmit HIV or to initiate T cell responses.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
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