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Dendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin/CD209 Is Abundant on Macrophages in the Normal Human Lymph Node and Is Not Required for Dendritic Cell Stimulation of the Mixed Leukocyte Reaction
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박채규 | - |
dc.date.accessioned | 2015-08-26T16:44:02Z | - |
dc.date.available | 2015-08-26T16:44:02Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114989 | - |
dc.description.abstract | The C-type lectin dendritic cell-specific ICAM 3-grabbing nonintegrin (DC-SIGN)/CD209 efficiently binds several pathogens, including HIV-1. DC-SIGN is expressed on monocyte-derived DCs in culture, and importantly, it is able to sequester HIV-1 within cells and facilitate transmission of virus to CD4+ T cells. To investigate DC-SIGN function, we have generated new mAbs. We report in this study that these and prior anti-DC-SIGN mAbs primarily label macrophages in the medullary sinuses of noninflamed human lymph node. In contrast, expression is not detected on most DCs in the T cell area, except for occasional cells. We also noted that IL-4 alone can induce expression of DC-SIGN in CD14+ monocytes and circulating blood DCs. However, blockade of DC-SIGN with Abs and DC-SIGN small interfering RNA did not result in a major reduction in the capacity of these DCs to transfer HIV to T cells, confirming significant DC-SIGN-independent mechanisms. The blocking approaches did reduce HIV-1 transmission by DC-SIGN-transfected cells by >90%. DC-SIGN blockade also did not reduce the ability of DCs to stimulate T cell proliferation in the MLR. These results indicate that DC-SIGN has the potential to contribute to macrophage function in normal human lymph node, and that DCs do not require DC-SIGN to transmit HIV or to initiate T cell responses. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 4265~4273 | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Monoclonal | - |
dc.subject.MESH | Cell Adhesion Molecules/antagonists & inhibitors | - |
dc.subject.MESH | Cell Adhesion Molecules/genetics | - |
dc.subject.MESH | Cell Adhesion Molecules/immunology | - |
dc.subject.MESH | Cell Adhesion Molecules/metabolism* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Clone Cells | - |
dc.subject.MESH | Dendritic Cells/immunology* | - |
dc.subject.MESH | Dendritic Cells/virology | - |
dc.subject.MESH | Dogs | - |
dc.subject.MESH | HIV-1/immunology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lectins, C-Type/antagonists & inhibitors | - |
dc.subject.MESH | Lectins, C-Type/genetics | - |
dc.subject.MESH | Lectins, C-Type/immunology | - |
dc.subject.MESH | Lectins, C-Type/metabolism* | - |
dc.subject.MESH | Lymph Nodes/cytology | - |
dc.subject.MESH | Lymph Nodes/metabolism* | - |
dc.subject.MESH | Lymphocyte Culture Test, Mixed | - |
dc.subject.MESH | Macrophages/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.subject.MESH | Receptors, Cell Surface/antagonists & inhibitors | - |
dc.subject.MESH | Receptors, Cell Surface/genetics | - |
dc.subject.MESH | Receptors, Cell Surface/immunology | - |
dc.subject.MESH | Receptors, Cell Surface/metabolism* | - |
dc.subject.MESH | Recombinant Proteins/genetics | - |
dc.subject.MESH | Recombinant Proteins/immunology | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.subject.MESH | T-Lymphocytes/virology | - |
dc.subject.MESH | Transfection | - |
dc.title | Dendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin/CD209 Is Abundant on Macrophages in the Normal Human Lymph Node and Is Not Required for Dendritic Cell Stimulation of the Mixed Leukocyte Reaction | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Angela Granelli-Piperno | - |
dc.contributor.googleauthor | Alla Pritsker | - |
dc.contributor.googleauthor | Ralph M. Steinman | - |
dc.contributor.googleauthor | Thomas M. Moran | - |
dc.contributor.googleauthor | Vincent Piguet | - |
dc.contributor.googleauthor | Christine Trumpfheller | - |
dc.contributor.googleauthor | Chae Gyu Park | - |
dc.contributor.googleauthor | Jean-Francois Arrighi | - |
dc.contributor.googleauthor | Irina Shimeliovich | - |
dc.contributor.googleauthor | Maggi Pack | - |
dc.identifier.doi | OAK-2005-02270 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01718 | - |
dc.relation.journalcode | J01450 | - |
dc.identifier.eissn | 1550-6606 | - |
dc.identifier.pmid | 16177066 | - |
dc.subject.keyword | 16177066 | - |
dc.contributor.alternativeName | Park, Chae Gyu | - |
dc.contributor.affiliatedAuthor | Park, Chae Gyu | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 175 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 4265 | - |
dc.citation.endPage | 4273 | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, Vol.175(7) : 4265-4273, 2005 | - |
dc.identifier.rimsid | 39364 | - |
dc.type.rims | ART | - |
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