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Dendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin/CD209 Is Abundant on Macrophages in the Normal Human Lymph Node and Is Not Required for Dendritic Cell Stimulation of the Mixed Leukocyte Reaction

DC FieldValueLanguage
dc.contributor.author박채규-
dc.date.accessioned2015-08-26T16:44:02Z-
dc.date.available2015-08-26T16:44:02Z-
dc.date.issued2005-
dc.identifier.issn0022-1767-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114989-
dc.description.abstractThe C-type lectin dendritic cell-specific ICAM 3-grabbing nonintegrin (DC-SIGN)/CD209 efficiently binds several pathogens, including HIV-1. DC-SIGN is expressed on monocyte-derived DCs in culture, and importantly, it is able to sequester HIV-1 within cells and facilitate transmission of virus to CD4+ T cells. To investigate DC-SIGN function, we have generated new mAbs. We report in this study that these and prior anti-DC-SIGN mAbs primarily label macrophages in the medullary sinuses of noninflamed human lymph node. In contrast, expression is not detected on most DCs in the T cell area, except for occasional cells. We also noted that IL-4 alone can induce expression of DC-SIGN in CD14+ monocytes and circulating blood DCs. However, blockade of DC-SIGN with Abs and DC-SIGN small interfering RNA did not result in a major reduction in the capacity of these DCs to transfer HIV to T cells, confirming significant DC-SIGN-independent mechanisms. The blocking approaches did reduce HIV-1 transmission by DC-SIGN-transfected cells by >90%. DC-SIGN blockade also did not reduce the ability of DCs to stimulate T cell proliferation in the MLR. These results indicate that DC-SIGN has the potential to contribute to macrophage function in normal human lymph node, and that DCs do not require DC-SIGN to transmit HIV or to initiate T cell responses.-
dc.description.statementOfResponsibilityopen-
dc.format.extent4265~4273-
dc.relation.isPartOfJOURNAL OF IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Monoclonal-
dc.subject.MESHCell Adhesion Molecules/antagonists & inhibitors-
dc.subject.MESHCell Adhesion Molecules/genetics-
dc.subject.MESHCell Adhesion Molecules/immunology-
dc.subject.MESHCell Adhesion Molecules/metabolism*-
dc.subject.MESHCell Line-
dc.subject.MESHClone Cells-
dc.subject.MESHDendritic Cells/immunology*-
dc.subject.MESHDendritic Cells/virology-
dc.subject.MESHDogs-
dc.subject.MESHHIV-1/immunology-
dc.subject.MESHHumans-
dc.subject.MESHLectins, C-Type/antagonists & inhibitors-
dc.subject.MESHLectins, C-Type/genetics-
dc.subject.MESHLectins, C-Type/immunology-
dc.subject.MESHLectins, C-Type/metabolism*-
dc.subject.MESHLymph Nodes/cytology-
dc.subject.MESHLymph Nodes/metabolism*-
dc.subject.MESHLymphocyte Culture Test, Mixed-
dc.subject.MESHMacrophages/metabolism*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHRNA, Small Interfering/metabolism-
dc.subject.MESHReceptors, Cell Surface/antagonists & inhibitors-
dc.subject.MESHReceptors, Cell Surface/genetics-
dc.subject.MESHReceptors, Cell Surface/immunology-
dc.subject.MESHReceptors, Cell Surface/metabolism*-
dc.subject.MESHRecombinant Proteins/genetics-
dc.subject.MESHRecombinant Proteins/immunology-
dc.subject.MESHRecombinant Proteins/metabolism-
dc.subject.MESHT-Lymphocytes/virology-
dc.subject.MESHTransfection-
dc.titleDendritic Cell-Specific Intercellular Adhesion Molecule 3-Grabbing Nonintegrin/CD209 Is Abundant on Macrophages in the Normal Human Lymph Node and Is Not Required for Dendritic Cell Stimulation of the Mixed Leukocyte Reaction-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorAngela Granelli-Piperno-
dc.contributor.googleauthorAlla Pritsker-
dc.contributor.googleauthorRalph M. Steinman-
dc.contributor.googleauthorThomas M. Moran-
dc.contributor.googleauthorVincent Piguet-
dc.contributor.googleauthorChristine Trumpfheller-
dc.contributor.googleauthorChae Gyu Park-
dc.contributor.googleauthorJean-Francois Arrighi-
dc.contributor.googleauthorIrina Shimeliovich-
dc.contributor.googleauthorMaggi Pack-
dc.identifier.doiOAK-2005-02270-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01718-
dc.relation.journalcodeJ01450-
dc.identifier.eissn1550-6606-
dc.identifier.pmid16177066-
dc.subject.keyword16177066-
dc.contributor.alternativeNamePark, Chae Gyu-
dc.contributor.affiliatedAuthorPark, Chae Gyu-
dc.rights.accessRightsfree-
dc.citation.volume175-
dc.citation.number7-
dc.citation.startPage4265-
dc.citation.endPage4273-
dc.identifier.bibliographicCitationJOURNAL OF IMMUNOLOGY, Vol.175(7) : 4265-4273, 2005-
dc.identifier.rimsid39364-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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