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Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds

DC FieldValueLanguage
dc.contributor.author손현주-
dc.contributor.author한동욱-
dc.contributor.author김학희-
dc.contributor.author박종철-
dc.contributor.author백현숙-
dc.date.accessioned2015-08-26T16:38:50Z-
dc.date.available2015-08-26T16:38:50Z-
dc.date.issued2005-
dc.identifier.issn1013-9826-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114846-
dc.description.abstractThe potential protective roles played by green tea compounds (GTPCs) against reactive oxygen species-induced oxidative stress in cultured fetal human dermal fibroblasts (fHDFs) were investigated according to cell viability measurement methods, such as fluorescence double staining followed by flow cytometry (FCM), MTT assay and crystal violet uptake. Oxidative stress was induced in the fHDFs, either by adding 50 mM H2O2 or by the action of 40 U/L xanthine oxidase (XO) in the presence of xanthine (250 µM). FCM analysis was the most suitable to show that both treatments produced a significant (p < 0.05) reduction in the fHDF viability, attributed to its high sensitivity. On the microscopic observations, the cell death with necrotic morphology was appreciably induced by both treatments. These oxidative stress-induced damages were significantly (p < 0.05) prevented by pre-incubating the fHDFs with 200 µg/ml GTPC for 1 h. These results suggest that GTPC can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent665~668-
dc.relation.isPartOfKey Engineering Materials-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHGreen Tea Polyphenolic Compounds-
dc.subject.MESHHuman Dermal Fibroblast-
dc.subject.MESHOxidative Stress-
dc.subject.MESHViability-
dc.titleProtection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학)-
dc.contributor.googleauthorDong Wook Han-
dc.contributor.googleauthorH.H. Kim-
dc.contributor.googleauthorJong Chul Park-
dc.contributor.googleauthorSuong Hyu Hyon-
dc.contributor.googleauthorKwon Yong Lee-
dc.contributor.googleauthorHyun Sook Baek-
dc.contributor.googleauthorHyun Joo Son-
dc.identifier.doi10.4028/www.scientific.net/KEM.288-289.665-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02001-
dc.contributor.localIdA04275-
dc.contributor.localIdA01095-
dc.contributor.localIdA01662-
dc.contributor.localIdA01843-
dc.relation.journalcodeJ01939-
dc.identifier.pmid10.4028/www.scientific.net/KEM.288-289.665-
dc.identifier.urlhttp://www.scientific.net/KEM.288-289.665-
dc.subject.keywordGreen Tea Polyphenolic Compounds-
dc.subject.keywordHuman Dermal Fibroblast-
dc.subject.keywordOxidative Stress-
dc.subject.keywordViability-
dc.contributor.alternativeNameSon, Hyun Joo-
dc.contributor.alternativeNameHan, Dong Wook-
dc.contributor.alternativeNameKim, Hak Hee-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameBaek, Hyun Sook-
dc.contributor.affiliatedAuthorSon, Hyun Joo-
dc.contributor.affiliatedAuthorHan, Dong Wook-
dc.contributor.affiliatedAuthorKim, Hak Hee-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorBaek, Hyun Sook-
dc.rights.accessRightsnot free-
dc.citation.volume288~289-
dc.citation.startPage665-
dc.citation.endPage668-
dc.identifier.bibliographicCitationKey Engineering Materials, Vol.288~289 : 665-668, 2005-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers

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