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Computer-Assisted Image Processing Techniques for Quantitative Analysis of Cell Migrations on Collagen-Coated Glass

Authors
 B.J. Park  ;  Seok Cheol Kim  ;  Jong Chul Park  ;  M. Aihara  ;  K. Takatori  ;  K.C. Nam  ;  Hyun Joo Son  ;  D.H. Lee 
Citation
 Key Engineering Materials, Vol.288~289 : 503-506, 2005 
Journal Title
 Key Engineering Materials 
ISSN
 1013-9826 
Issue Date
2005
Abstract
In this study, a computer-assisted cell tracking system including an automatic image processing program for rapid and precise analysis of cell migration in various conditions was self-designed and L-929 cell migration on the glass coated with type I collagen was examined using this cell tracking system. Furthermore, computer-based image processing software, with the capture program to choose the capture interval and period, and analysis techniques were developed for quantitative analysis of the cell migration on extracellular matrices. The results showed that the migration speed of L-929 cells on the collagen-coated glass was significantly (p < 0.05) increased compared to the non-coated control. On the morphological observations, it was showed that the cells on the collagen-coated glass looked much healthier than those on the control. These results suggested that this cell tracking system would provide tools for the analysis of cell migration in various in vitro conditions and might be effective enough to evaluate various biological events including embryonic development as well as physiological and pathological tissue reorganization.
Full Text
http://www.scientific.net/KEM.288-289.503
DOI
10.4028/www.scientific.net/KEM.288-289.503
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Son, Hyun Joo(손현주)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114844
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