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Gene knockdown by large circular antisense for high-throughput functional genomics

Authors
 Yun-Han Lee  ;  Ik-Jae Moon  ;  Jong-Gu Park  ;  Young-Ho Kim  ;  Young-Bae Seu  ;  Jong-Wook Park  ;  Koo-Jeong Kang  ;  Yong-Joo Kim  ;  Seok-Yong Uhm  ;  Kil-Hwan Han  ;  Jeong-Hoh Park  ;  Bin Hur 
Citation
 NATURE BIOTECHNOLOGY, Vol.23(5) : 591-599, 2005 
Journal Title
NATURE BIOTECHNOLOGY
ISSN
 1087-0156 
Issue Date
2005
MeSH
Animals ; Bacteriophage M13/genetics ; Cell Line, Tumor ; Chromosome Mapping/methods* ; DNA, Antisense/genetics* ; Gene Expression Profiling/methods* ; Gene Silencing* ; Gene Targeting/methods* ; Genomics/methods* ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism* ; Mice ; Neoplasm Proteins/genetics ; Neoplasm Proteins/metabolism*
Keywords
15867911
Abstract
Single-stranded genomic DNA of recombinant M13 phages was tested as an antisense molecule and examined for its usefulness in high-throughput functional genomics. cDNA fragments of various genes (TNF-alpha, c-myc, c-myb, cdk2 and cdk4) were independently cloned into phagemid vectors. Using the life cycle of M13 bacteriophages, large circular (LC)-molecules, antisense to their respective genes, were prepared from the culture supernatant of bacterial transformants. LC-antisense molecules exhibited enhanced stability, target specificity and no need for target-site searches. High-throughput functional genomics was then attempted with an LC-antisense library, which was generated by using a phagemid vector that incorporated a unidirectional subtracted cDNA library derived from liver cancer tissue. We identified 56 genes involved in the growth of these cells. These results indicate that an antisense sequence as a part of single-stranded LC-genomic DNA of recombinant M13 phages exhibits effective antisense activity, and may have potential for high-throughput functional genomics.
Full Text
http://www.nature.com/nbt/journal/v23/n5/full/nbt1089.html
DOI
10.1038/nbt1089
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Han(이윤한)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114787
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