Cited 9 times in
Gene knockdown by large circular antisense for high-throughput functional genomics
DC Field | Value | Language |
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dc.contributor.author | 이윤한 | - |
dc.date.accessioned | 2015-08-26T16:36:43Z | - |
dc.date.available | 2015-08-26T16:36:43Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1087-0156 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114787 | - |
dc.description.abstract | Single-stranded genomic DNA of recombinant M13 phages was tested as an antisense molecule and examined for its usefulness in high-throughput functional genomics. cDNA fragments of various genes (TNF-alpha, c-myc, c-myb, cdk2 and cdk4) were independently cloned into phagemid vectors. Using the life cycle of M13 bacteriophages, large circular (LC)-molecules, antisense to their respective genes, were prepared from the culture supernatant of bacterial transformants. LC-antisense molecules exhibited enhanced stability, target specificity and no need for target-site searches. High-throughput functional genomics was then attempted with an LC-antisense library, which was generated by using a phagemid vector that incorporated a unidirectional subtracted cDNA library derived from liver cancer tissue. We identified 56 genes involved in the growth of these cells. These results indicate that an antisense sequence as a part of single-stranded LC-genomic DNA of recombinant M13 phages exhibits effective antisense activity, and may have potential for high-throughput functional genomics. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 591~599 | - |
dc.relation.isPartOf | NATURE BIOTECHNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bacteriophage M13/genetics | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Chromosome Mapping/methods* | - |
dc.subject.MESH | DNA, Antisense/genetics* | - |
dc.subject.MESH | Gene Expression Profiling/methods* | - |
dc.subject.MESH | Gene Silencing* | - |
dc.subject.MESH | Gene Targeting/methods* | - |
dc.subject.MESH | Genomics/methods* | - |
dc.subject.MESH | Liver Neoplasms/genetics | - |
dc.subject.MESH | Liver Neoplasms/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neoplasm Proteins/genetics | - |
dc.subject.MESH | Neoplasm Proteins/metabolism* | - |
dc.title | Gene knockdown by large circular antisense for high-throughput functional genomics | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Yun-Han Lee | - |
dc.contributor.googleauthor | Ik-Jae Moon | - |
dc.contributor.googleauthor | Jong-Gu Park | - |
dc.contributor.googleauthor | Young-Ho Kim | - |
dc.contributor.googleauthor | Young-Bae Seu | - |
dc.contributor.googleauthor | Jong-Wook Park | - |
dc.contributor.googleauthor | Koo-Jeong Kang | - |
dc.contributor.googleauthor | Yong-Joo Kim | - |
dc.contributor.googleauthor | Seok-Yong Uhm | - |
dc.contributor.googleauthor | Kil-Hwan Han | - |
dc.contributor.googleauthor | Jeong-Hoh Park | - |
dc.contributor.googleauthor | Bin Hur | - |
dc.identifier.doi | 10.1038/nbt1089 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03029 | - |
dc.relation.journalcode | J02290 | - |
dc.identifier.eissn | 1546-1696 | - |
dc.identifier.pmid | 15867911 | - |
dc.identifier.url | http://www.nature.com/nbt/journal/v23/n5/full/nbt1089.html | - |
dc.subject.keyword | 15867911 | - |
dc.contributor.alternativeName | Lee, Yun Han | - |
dc.contributor.affiliatedAuthor | Lee, Yun Han | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 23 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 591 | - |
dc.citation.endPage | 599 | - |
dc.identifier.bibliographicCitation | NATURE BIOTECHNOLOGY, Vol.23(5) : 591-599, 2005 | - |
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