Cited 3 times in
Expression of Na+/H+ exchanger isoforms in normal human nasal epithelial cells and functional activity of Na+/H+ exchanger 1 in intracellular pH regulation
DC Field | Value | Language |
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dc.contributor.author | 이정권 | - |
dc.contributor.author | 최재영 | - |
dc.contributor.author | 김창훈 | - |
dc.contributor.author | 신지현 | - |
dc.contributor.author | 유종범 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이민구 | - |
dc.date.accessioned | 2015-08-26T16:34:28Z | - |
dc.date.available | 2015-08-26T16:34:28Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0001-6489 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114727 | - |
dc.description.abstract | CONCLUSIONS: Both the mRNA and protein of NHE1, -2 and -3 were expressed in NHNE cells. NHE1 is a major NHE isoform which is expressed in the basolateral membranes of NHNE cells. OBJECTIVE: Na+/H+ exchangers are ubiquitous plasma membrane transport proteins implicated in the maintenance of intracellular pH. The aim of this study was to examine the expression of Na+/H+ exchangers as a function of the differentiation of normal human nasal epithelial (NHNE) cells. In addition, we investigated the functional activity of the Na+/H+ exchanger 1 (NHE1) in basolateral membranes. MATERIAL AND METHODS: Cultured passage-2 NHNE cells were used. RNA and histological samples were collected on the day of confluence and on the 7th, 14th and 28th days after confluence in order to determine the effects of time. Cell lysates were collected on the day of confluence to investigate the presence of the proteins. Reverse transcriptase polymerase chain reaction and Western blotting were performed to investigate the presence of mRNA and protein, respectively. The functional activity of NHE1 was examined using 3-methylsulfonyl-4-piperidinobenzoyl guanidine methanesulfonate (HOE694), an NHE1-specific inhibitor, on the day of confluence. RESULTS: The NHE1 mRNA expression level did not change as a function of differentiation. However, the NHE2 mRNA expression levels increased on the 7th, 14th and 28th days after confluence. The NHE3 mRNA expression levels increased on the 14th and 28th days after confluence. Western blot analysis confirmed the expression of NHE1 (91 kDa), NHE2 (90 kDa) and NHE3 (93 kDa). In addition, HOE694 inhibited basolateral NHE activity by 68% at 1 microM and by 85% at 5 microM in the NHNE cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | ACTA OTO-LARYNGOLOGICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cation Transport Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Cation Transport Proteins/metabolism* | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Epithelial Cells/pathology | - |
dc.subject.MESH | Guanidines/pharmacology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen-Ion Concentration | - |
dc.subject.MESH | Intracellular Fluid/chemistry* | - |
dc.subject.MESH | Membrane Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Membrane Proteins/metabolism* | - |
dc.subject.MESH | Nasal Mucosa/cytology* | - |
dc.subject.MESH | Protein Isoforms/metabolism | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Sodium-Hydrogen Exchanger 1 | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/antagonists & inhibitors | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/metabolism* | - |
dc.subject.MESH | Sulfones/pharmacology | - |
dc.title | Expression of Na+/H+ exchanger isoforms in normal human nasal epithelial cells and functional activity of Na+/H+ exchanger 1 in intracellular pH regulation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Ji-Hyun Shin | - |
dc.contributor.googleauthor | Wan Namkung | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.contributor.googleauthor | Jeung-Gweon Lee | - |
dc.contributor.googleauthor | Kyung-Dong Lee | - |
dc.contributor.googleauthor | Jong-Bum Yoo | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.contributor.googleauthor | Chang-Hoon Kim | - |
dc.identifier.doi | 10.1080/00016480410022976 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04173 | - |
dc.contributor.localId | A01050 | - |
dc.contributor.localId | A03095 | - |
dc.contributor.localId | A02163 | - |
dc.contributor.localId | A02510 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A02781 | - |
dc.relation.journalcode | J00028 | - |
dc.identifier.eissn | 1651-2251 | - |
dc.identifier.pmid | 15966699 | - |
dc.identifier.url | http://informahealthcare.com/doi/abs/10.1080/00016480410022976 | - |
dc.subject.keyword | 15966699 | - |
dc.contributor.alternativeName | Lee, Jeung Gweon | - |
dc.contributor.alternativeName | Choi, Jae Young | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Shin, Ji Hyun | - |
dc.contributor.alternativeName | Yoo, Jong Bum | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Choi, Jae Young | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Jeung Gweon | - |
dc.contributor.affiliatedAuthor | Shin, Ji Hyun | - |
dc.contributor.affiliatedAuthor | Yoo, Jong Bum | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 125 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 286 | - |
dc.citation.endPage | 292 | - |
dc.identifier.bibliographicCitation | ACTA OTO-LARYNGOLOGICA, Vol.125(3) : 286-292, 2005 | - |
dc.identifier.rimsid | 46079 | - |
dc.type.rims | ART | - |
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