Cited 29 times in
Inhibitory effect of procyanidin oligomer from elm cortex on the matrix metalloproteinases and proteases of periodontopathogens
DC Field | Value | Language |
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dc.contributor.author | 김종관 | - |
dc.contributor.author | 유윤정 | - |
dc.date.accessioned | 2015-07-15T17:15:01Z | - |
dc.date.available | 2015-07-15T17:15:01Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114531 | - |
dc.description.abstract | OBJECTIVES: The purpose of this study was to evaluate a partially purified extract (elm extract) from the Ulmi cortex (Ulmi macrocarpa Hance) and its active ingredient, a mix of procyanidin oligomers (3 to 12 flavan-3-ol monomers, an average molecular weight of 1,518 with an average polymerization degree of 5.3) for a possible inhibitory effect against proteases. BACKGROUND: Host-derived matrix metalloproteinases (MMPs) and bacterial proteases play important roles in the gingival tissue destruction that is a characteristic of periodontitis. The inhibitors of these proteases may be developed into therapeutic agents against periodontitis. METHODS: The inhibitory effects were assessed by gelatin zymography. The MMPs tested were originated from the gingival crevicular fluid (GCF) of adult periodontitis patients and from the conditioned media of cultured periodontal ligament (PDL) cells, which provided the proMMP-2 and activated MMP-2 when treated with a periodontopathogen, Treponema lecithinolyticum. Bacterial enzymes tested were secreted forms from two major periodontopathogens, Porphyromonas gingivalis and Treponema denticola. In addition, the inhibitory effects on trypsin-like enzymes from these two periodontopathogens were assayed by the n-benzoyl-DL-arginine-naphthylamide (BANA) test. RESULTS: The elm extract and the procyanidin oligomer (100-1,000 microg/ml) exhibited potent inhibitory effects on the MMPs in GCF (chiefly MMP-8 and MMP-9), the pro and active forms of MMP-2, and secreted and trypsin-like enzymes from T. denticola and P. gingivalis. CONCLUSIONS: These results suggest that elm cortex should be considered as a potential agent against periodontal diseases, due to its inhibitory action on MMPs and the proteases of periodontopathogens. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 282~289 | - |
dc.relation.isPartOf | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Antioxidants/pharmacology* | - |
dc.subject.MESH | Biflavonoids* | - |
dc.subject.MESH | Catechin/pharmacology* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology* | - |
dc.subject.MESH | Gingival Crevicular Fluid/enzymology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Matrix Metalloproteinase Inhibitors* | - |
dc.subject.MESH | Periodontal Ligament/enzymology | - |
dc.subject.MESH | Periodontitis/enzymology | - |
dc.subject.MESH | Periodontitis/microbiology | - |
dc.subject.MESH | Plant Extracts/pharmacology* | - |
dc.subject.MESH | Porphyromonas gingivalis/drug effects | - |
dc.subject.MESH | Porphyromonas gingivalis/enzymology* | - |
dc.subject.MESH | Proanthocyanidins* | - |
dc.subject.MESH | Protease Inhibitors/pharmacology* | - |
dc.subject.MESH | Statistics, Nonparametric | - |
dc.subject.MESH | Treponema/drug effects | - |
dc.subject.MESH | Treponema/enzymology* | - |
dc.subject.MESH | Trypsin Inhibitors/pharmacology | - |
dc.subject.MESH | Ulmus* | - |
dc.title | Inhibitory effect of procyanidin oligomer from elm cortex on the matrix metalloproteinases and proteases of periodontopathogens | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Si-Eun Song | - |
dc.contributor.googleauthor | Bong-Kyu Choi | - |
dc.contributor.googleauthor | Chong-Kwan Kim | - |
dc.contributor.googleauthor | Seok-Sun Roh | - |
dc.contributor.googleauthor | Sang-Ki Park | - |
dc.contributor.googleauthor | Moon-Moo Kim | - |
dc.contributor.googleauthor | Yun-Jung Yoo | - |
dc.contributor.googleauthor | Sang-Nyun Kim | - |
dc.identifier.doi | 10.1034/j.1600-0765.2003.02604.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02490 | - |
dc.contributor.localId | A00914 | - |
dc.relation.journalcode | J01696 | - |
dc.identifier.eissn | 1600-0765 | - |
dc.identifier.pmid | 12753366 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1034/j.1600-0765.2003.02604.x/abstract | - |
dc.subject.keyword | elm cortex | - |
dc.subject.keyword | procyanidin oligomer | - |
dc.subject.keyword | matrix metalloproteinases | - |
dc.subject.keyword | periodotitis | - |
dc.contributor.alternativeName | Kim, Chong Kwan | - |
dc.contributor.alternativeName | Yoo, Yun Jung | - |
dc.contributor.affiliatedAuthor | Yoo, Yun Jung | - |
dc.contributor.affiliatedAuthor | Kim, Chong Kwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 38 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 282 | - |
dc.citation.endPage | 289 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PERIODONTAL RESEARCH, Vol.38(3) : 282-289, 2003 | - |
dc.identifier.rimsid | 43912 | - |
dc.type.rims | ART | - |
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