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Heterospecific Expression of the Bacillus subtilis Cell Shape Determination Genes mreBCD in Escherichia coli

Authors
 Joong-Chul Lee  ;  Jeong-Heon Cha  ;  George C. Stewart  ;  Dennis B. Zerbv 
Citation
 CURRENT MICROBIOLOGY, Vol.47(2) : 146-152, 2003 
Journal Title
CURRENT MICROBIOLOGY
ISSN
 0343-8651 
Issue Date
2003
MeSH
Alkaline Phosphatase ; Bacillus subtilis/cytology* ; Bacillus subtilis/genetics ; Bacillus subtilis/metabolism ; Bacterial Proteins/genetics* ; Bacterial Proteins/metabolism ; Blotting, Western ; DNA Transposable Elements ; Escherichia coli/cytology ; Escherichia coli/genetics ; Escherichia coli/metabolism* ; Escherichia coli Proteins* ; Gene Expression Regulation, Bacterial* ; Microscopy, Fluorescence ; Mutagenesis
Keywords
bacillus subtilis ; carboxy ; peptidoglycan ; determination Gene ; amino terminus
Abstract
The divIVB operon of Bacillus subtilis includes the cell shape-associated mre genes, including the membrane-associated proteins MreC and MreD. TnphoA mutagenesis was utilized to analyze a topological model for MreC. MreC has a short cytoplasmic amino terminus, a single membrane-spanning domain, and a large carboxy terminal domain which lies externally to the outer leaflet of the cell membrane. Expression of the B. subtilis MreB protein, or the Mre C and D proteins, results in a morphological conversion of the Escherichia coli host cells from a rod to a roughly spherical cell, morphologically similar to mre-negative mutants of E. coli. Immunolocalization of the MreC protein in B. subtilis revealed that this protein is found at the midcell division site of the bacterial cells, consistent with the postulated role of the Mre proteins in the regulation of septum-specific peptidoglycan synthesis.
Full Text
http://link.springer.com/article/10.1007/s00284-002-3937-9
DOI
10.1007/s00284-002-3937-9
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Cha, Jeong Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114509
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