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A Novel Gene, Jpk, Induces Apoptosis in F9 Murine Teratocarcinoma Cell through ROS Generation

Authors
 KYOUNG-AH KONG  ;  SUNGDO PARK  ;  MYOUNG HEE KIM  ;  HYOUNGWOO PARK 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 433-436, 2003 
Journal Title
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
ISSN
 0077-8923 
Issue Date
2003
MeSH
Animals ; Cell Line, Tumor ; Gastrula/physiology ; Gene Expression Regulation, Developmental ; Intracellular Membranes/physiology ; Membrane Potentials ; Mice ; Mitochondria/physiology* ; Reactive Oxygen Species/metabolism* ; Teratocarcinoma
Keywords
Jpk ; EGFP ; apoptosis ; ROS ; mitochondrial transmembrane potential
Abstract
A novel gene Jpk (Jopock) has been originally isolated through yeast 1 hybridization technique as a trans-acting factor interacting with the position-specific regulatory element of a murine Hoxa-7. Northern analysis revealed that the Jpk was expressed at day 7.0 post coitum (p.c.) during early gastrulation. Previously it has been shown that a trace amount of JPK protein led bacterial cells to death. In eukaryotic F9 cells, Jpk also led the cell to death-generating DNA ladder: fewer than 50% of the cells survived after 72-h transfection. Flow cytometric analysis with cells stained with each Annexin V/7-amino-actinomycin D (7-AAD), MitoTracker, and hydroethidine (HE) revealed that Jpk induced apoptotic cell death in a time-dependent manner, reduced mitochondrial membrane potential, and increased ROS (reactive oxygen species) production, respectively. Additionally, Jpk seemed to regulate the Bcl family at the transcriptional level when RT-PCR was performed. Although the precise mechanism is not clear, these results altogether suggest that Jpk is a potent inducer of apoptosis through generation of ROS as well as concomitant reduction of mitochondrial membrane potential.
Full Text
http://onlinelibrary.wiley.com/doi/10.1196/annals.1299.078/abstract
DOI
10.1196/annals.1299.078
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kong, Kyoung Ah(공경아)
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Park, Hyoung Woo(박형우) ORCID logo https://orcid.org/0000-0002-5341-9249
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114456
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