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변이 연골 과소기질 단백질이 Swarm Rat 연골육종세포주의 세포외 기질 생성에 미치는 영향

Other Titles
 Effects of Mutant Cartilage Oligomeric Matrix Protein on the Synthesis of Extracellular Matrix in the Swarm Rat Chondrosarcoma Cell Line 
 김현우  ;  박문수  ;  박희완  ;  한수봉  ;  신규호  ;  공선영  ;  송우석 
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.6(1) : 47-54, 2003 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
Issue Date
Mutant COMP ; Extracellular matrix
Mutant COMP ; Extracellular matrix
Purpose: To investigate the effects of mutated cartilage oligomeric matrix protein (COMP) on the synthesis of cartilage-specific major matrix proteins of Swarm rat chondrosarcoma chondrocytes. Materials and Methods: The Swarm rat chondrosarcoma chondrocytes transfected with chimeric construct consisting of a mutant gene of human COMP and an amino acid FLAG tag sequence were cultured in agarose gel. Formation of extracellular proteoglycan and type-Ⅱ collagen of the cells were evaluated by immunohistochemical staining and measuring 35S-sulfate incorporation. Results: No difference was observed in type-ll collagen detection among the cell line expressing mutant COMP and control cell lines. Histochemical staining of sulfated proteoglycans with safranin-0 showed lower amounts of proteoglycans were incorporated into the extracellular matrix of chondrocytes transfected with mutant gene. 35S-sulfate incorporation into the cell/matrix fractions demonstrates marked lower radiolabel incorporation compared to control cells. Conclusion: Mutation of COMP impacts the processing of proteoglycans rather than type-Ⅱ collagen in three-dimensional culture of Swarm rat chondrosarcoma chondrocytes.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Park, Moon Soo(박문수)
Park, Hui Wan(박희완)
Song, Woo Suk(송우석)
Shin, Kyoo Ho(신규호)
Hahn, Soo Bong(한수봉)
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