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변이 연골 과소기질 단백질이 Swarm Rat 연골육종세포주의 세포외 기질 생성에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김현우 | - |
dc.contributor.author | 박문수 | - |
dc.contributor.author | 박희완 | - |
dc.contributor.author | 송우석 | - |
dc.contributor.author | 신규호 | - |
dc.contributor.author | 한수봉 | - |
dc.date.accessioned | 2015-07-15T17:12:34Z | - |
dc.date.available | 2015-07-15T17:12:34Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1226-8933 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114448 | - |
dc.description.abstract | Purpose: To investigate the effects of mutated cartilage oligomeric matrix protein (COMP) on the synthesis of cartilage-specific major matrix proteins of Swarm rat chondrosarcoma chondrocytes. Materials and Methods: The Swarm rat chondrosarcoma chondrocytes transfected with chimeric construct consisting of a mutant gene of human COMP and an amino acid FLAG tag sequence were cultured in agarose gel. Formation of extracellular proteoglycan and type-Ⅱ collagen of the cells were evaluated by immunohistochemical staining and measuring 35S-sulfate incorporation. Results: No difference was observed in type-ll collagen detection among the cell line expressing mutant COMP and control cell lines. Histochemical staining of sulfated proteoglycans with safranin-0 showed lower amounts of proteoglycans were incorporated into the extracellular matrix of chondrocytes transfected with mutant gene. 35S-sulfate incorporation into the cell/matrix fractions demonstrates marked lower radiolabel incorporation compared to control cells. Conclusion: Mutation of COMP impacts the processing of proteoglycans rather than type-Ⅱ collagen in three-dimensional culture of Swarm rat chondrosarcoma chondrocytes. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Mutant COMP | - |
dc.subject.MESH | Extracellular matrix | - |
dc.title | 변이 연골 과소기질 단백질이 Swarm Rat 연골육종세포주의 세포외 기질 생성에 미치는 영향 | - |
dc.title.alternative | Effects of Mutant Cartilage Oligomeric Matrix Protein on the Synthesis of Extracellular Matrix in the Swarm Rat Chondrosarcoma Cell Line | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 김현우 | - |
dc.contributor.googleauthor | 박문수 | - |
dc.contributor.googleauthor | 박희완 | - |
dc.contributor.googleauthor | 한수봉 | - |
dc.contributor.googleauthor | 신규호 | - |
dc.contributor.googleauthor | 공선영 | - |
dc.contributor.googleauthor | 송우석 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01124 | - |
dc.contributor.localId | A01459 | - |
dc.contributor.localId | A01777 | - |
dc.contributor.localId | A02042 | - |
dc.contributor.localId | A02086 | - |
dc.contributor.localId | A04293 | - |
dc.relation.journalcode | J01526 | - |
dc.subject.keyword | Mutant COMP | - |
dc.subject.keyword | Extracellular matrix | - |
dc.contributor.alternativeName | Kim, Hyun Woo | - |
dc.contributor.alternativeName | Park, Moon Soo | - |
dc.contributor.alternativeName | Park, Hui Wan | - |
dc.contributor.alternativeName | Song, Woo Suk | - |
dc.contributor.alternativeName | Shin, Kyoo Ho | - |
dc.contributor.alternativeName | Hahn, Soo Bong | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Woo | - |
dc.contributor.affiliatedAuthor | Park, Moon Soo | - |
dc.contributor.affiliatedAuthor | Park, Hui Wan | - |
dc.contributor.affiliatedAuthor | Song, Woo Suk | - |
dc.contributor.affiliatedAuthor | Shin, Kyoo Ho | - |
dc.contributor.affiliatedAuthor | Hahn, Soo Bong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 6 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 47 | - |
dc.citation.endPage | 54 | - |
dc.identifier.bibliographicCitation | Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.6(1) : 47-54, 2003 | - |
dc.identifier.rimsid | 43850 | - |
dc.type.rims | ART | - |
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