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Apoptosis in growing hair follicles following gamma-irradiation and application for the evaluation of radioprotective agents

Authors
 Kim SH  ;  Kim SR  ;  Jo SK  ;  Kim TH  ;  Lee YS  ;  Ryu SY  ;  Oh H  ;  Lee HJ 
Citation
 IN VIVO, Vol.17(2) : 211-214, 2003 
Journal Title
IN VIVO
ISSN
 0258-851X 
Issue Date
2003
MeSH
Animals ; Apoptosis/radiation effects* ; Camellia sinensis/chemistry ; Cell Count ; Ditiocarb/pharmacology* ; Dose-Response Relationship, Radiation ; Gamma Rays ; Hair Follicle/pathology ; Hair Follicle/radiation effects* ; In Situ Nick-End Labeling ; Mice ; Mice, Inbred ICR ; Plant Extracts/chemistry ; Plant Extracts/pharmacology* ; Radiation-Protective Agents/pharmacology* ; Tea/chemistry
Keywords
12792989
Abstract
The usefulness of apoptotic fragments assay for investigating the radiation response of hair follicles and evaluation of radioprotective agents was examined in ICR mice. The extent of changes following 100 cGy (1000 cGy/min) was studied at 0, 2, 4, 8, 12, 16 or 20 hours after exposure. The maximal frequency was found 12 hours after exposure. The mice that received 50, 100, 200, 400 or 800 cGy of gamma-rays were examined 12 hours after irradiation. Measurements performed after gamma-ray irradiation showed a dose-related increase in apoptotic cells in each mouse studied. The dose-response curves were analyzed with a linear-quadratic model: the frequency (number per follicle) of apoptotic cells in the hair follicle was y = (0.05527 +/- 0.009574) D + (-0.00001988 +/- 0.00001337) D2 + 0.227 (r2 = 0.964, D = 100 cGy). The frequency of radiation (100 cGy)-induced apoptosis in hair follicles was reduced by pretreatment of diethyldithiocarbamate (DDC, i.p. at 30 minutes before irradiation, p < 0.05) or green tea (i.p. at 12 and 36 hours before irradiation, p < 0.01). From these results, it is thought that this model will be useful in the detection of radiation response and radioprotective agents.
DOI
OAK-2003-01227
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Sil(이윤실)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114387
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