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Characterization of a new integron containing VIM-2, a metallo- β-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae

Authors
 Hyuk Jin Choi  ;  Seok Hoon Jeong  ;  Tae Sik Jeong  ;  Sang Wook Lee  ;  Byung Hoon Han  ;  Kil Ho Park  ;  Jeong Man Kim  ;  Sang Hee Lee  ;  Jong Hwa Yum  ;  Yunsop Chong  ;  Kyungwon Lee 
Citation
 JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, Vol.51(2) : 397-400, 2003 
Journal Title
 JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 
ISSN
 0305-7453 
Issue Date
2003
Abstract
We report the first description of a new integron containing bla(VIM-2), a metallo-beta-lactamase gene from Enterobacter cloacae KU680, which was isolated from peritoneal fluid of a liver cirrhosis patient in South Korea. Antibiotic susceptibility testing, and modified Hodge and EDTA-disc synergy tests, were carried out to screen for metallo-beta-lactamase-producing strains. PCR and sequence analysis were used to identify and analyse the bla(VIM-2)-containing integron. The isolate was resistant to most beta-lactams, including imipenem, and demonstrated a positive modified Hodge and EDTA-disc synergy test, which are findings suggesting a metallo-beta-lactamase. Preliminary PCR-based experiments detected the metallo-beta-lactamase gene bla(VIM-2). Sequencing of the 4392 bp cloned PCR amplicon, containing the gene cassette bla(VIM-2), revealed the structure of the class 1 integron. The integron also contained additional insert gene cassettes, aadA, and unknown open reading frames 'orfII' and 'orfIII'. To the best of our knowledge, this is the first time that this metallo-beta-lactamase gene has been detected in E. cloacae.
Files in This Item:
T200305979.pdf Download
DOI
10.1093/jac/dkg047
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Institute of Bacterial Resistance (세균내성연구소) > 1. Journal Papers
Yonsei Authors
Yum, Jong Hwa(염종화)
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114257
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