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Inhibition of Rho-Associated Kinase Reduces MLC20 Phosphorylation and Contractility of Intact Myometrium and Attenuates Agonist-Induced Ca2+ Sensitization of Force of Permeabilized Rat Myometrium

Authors
 Jae-Hwan OH  ;  Sung-Kyung YOU  ;  Young-Ho LEE  ;  Michael J. TAGGART  ;  Seong-chun KWON  ;  Duck-Sun AHN  ;  Mi-Kyung HWANG 
Citation
 JOURNAL OF VETERINARY MEDICAL SCIENCE, Vol.65(1) : 43-50, 2003 
Journal Title
 JOURNAL OF VETERINARY MEDICAL SCIENCE 
ISSN
 0916-7250 
Issue Date
2003
MeSH
Amides/pharmacology* ; Animals ; Calcium/metabolism* ; Carbachol/pharmacology ; Cholinergic Agents/pharmacology ; Enzyme Inhibitors/pharmacology ; Female ; Intracellular Signaling Peptides and Proteins ; Myometrium/drug effects* ; Myometrium/metabolism ; Myometrium/physiology ; Myosin Light Chains/metabolism* ; Naphthalenes/pharmacology ; Permeability ; Phosphorylation/drug effects ; Pregnancy ; Protein-Serine-Threonine Kinases/antagonists & inhibitors* ; Protein-Serine-Threonine Kinases/metabolism ; Pyridines/pharmacology* ; Rats ; Rats, Sprague-Dawley ; Uterine Contraction/drug effects* ; rho-Associated Kinases
Keywords
12576703
Abstract
The role of rhoA/rho-associated kinase (ROK) signaling pathways in agonist-induced contraction of the rat myometrium was investigated. We measured the [Ca2+]i-force relationship, phosphorylation of myosin regulatory light chains (MLC20) in intact tissue and the Ca2+-sensitization of force in permeabilized myometrial cells of rat. In measurements of the relationship between [Ca2+]i and tension in intact tissue, Y-27632, a ROK inhibitor, significantly attenuated the carbachol-induced contraction without changing [Ca 2+]i. Phosphorylation of MLC20 was increased by carbachol and this increased phosphorylation was blocked by treatment of tissue with Y-27632. In tension measurements of single hyperpermeable cells, carbachol evoked sustained contraction at constant pCa 6.7 and these agonist-induced contractions were decreased by treatment with Y-27632. These results suggest that activation of a ROK-mediated signaling pathway(s) plays an important role in agonist-induced alterations in MLC20 phosphorylation and force of rat myometrium.
Files in This Item:
T200305378.pdf Download
DOI
10.1292/jvms.65.43
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Lee, Young Ho(이영호) ORCID logo https://orcid.org/0000-0002-5749-1045
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114087
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