Cited 16 times in
Inhibition of Rho-Associated Kinase Reduces MLC20 Phosphorylation and Contractility of Intact Myometrium and Attenuates Agonist-Induced Ca2+ Sensitization of Force of Permeabilized Rat Myometrium
DC Field | Value | Language |
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dc.contributor.author | 이영호 | - |
dc.date.accessioned | 2015-07-15T17:01:54Z | - |
dc.date.available | 2015-07-15T17:01:54Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0916-7250 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114087 | - |
dc.description.abstract | The role of rhoA/rho-associated kinase (ROK) signaling pathways in agonist-induced contraction of the rat myometrium was investigated. We measured the [Ca2+]i-force relationship, phosphorylation of myosin regulatory light chains (MLC20) in intact tissue and the Ca2+-sensitization of force in permeabilized myometrial cells of rat. In measurements of the relationship between [Ca2+]i and tension in intact tissue, Y-27632, a ROK inhibitor, significantly attenuated the carbachol-induced contraction without changing [Ca 2+]i. Phosphorylation of MLC20 was increased by carbachol and this increased phosphorylation was blocked by treatment of tissue with Y-27632. In tension measurements of single hyperpermeable cells, carbachol evoked sustained contraction at constant pCa 6.7 and these agonist-induced contractions were decreased by treatment with Y-27632. These results suggest that activation of a ROK-mediated signaling pathway(s) plays an important role in agonist-induced alterations in MLC20 phosphorylation and force of rat myometrium. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 43~50 | - |
dc.relation.isPartOf | JOURNAL OF VETERINARY MEDICAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amides/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium/metabolism* | - |
dc.subject.MESH | Carbachol/pharmacology | - |
dc.subject.MESH | Cholinergic Agents/pharmacology | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Intracellular Signaling Peptides and Proteins | - |
dc.subject.MESH | Myometrium/drug effects* | - |
dc.subject.MESH | Myometrium/metabolism | - |
dc.subject.MESH | Myometrium/physiology | - |
dc.subject.MESH | Myosin Light Chains/metabolism* | - |
dc.subject.MESH | Naphthalenes/pharmacology | - |
dc.subject.MESH | Permeability | - |
dc.subject.MESH | Phosphorylation/drug effects | - |
dc.subject.MESH | Pregnancy | - |
dc.subject.MESH | Protein-Serine-Threonine Kinases/antagonists & inhibitors* | - |
dc.subject.MESH | Protein-Serine-Threonine Kinases/metabolism | - |
dc.subject.MESH | Pyridines/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Uterine Contraction/drug effects* | - |
dc.subject.MESH | rho-Associated Kinases | - |
dc.title | Inhibition of Rho-Associated Kinase Reduces MLC20 Phosphorylation and Contractility of Intact Myometrium and Attenuates Agonist-Induced Ca2+ Sensitization of Force of Permeabilized Rat Myometrium | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Jae-Hwan OH | - |
dc.contributor.googleauthor | Sung-Kyung YOU | - |
dc.contributor.googleauthor | Young-Ho LEE | - |
dc.contributor.googleauthor | Michael J. TAGGART | - |
dc.contributor.googleauthor | Seong-chun KWON | - |
dc.contributor.googleauthor | Duck-Sun AHN | - |
dc.contributor.googleauthor | Mi-Kyung HWANG | - |
dc.identifier.doi | 10.1292/jvms.65.43 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02968 | - |
dc.relation.journalcode | J01926 | - |
dc.identifier.eissn | 1347-7439 | - |
dc.identifier.pmid | 12576703 | - |
dc.subject.keyword | 12576703 | - |
dc.contributor.alternativeName | Lee, Young Ho | - |
dc.contributor.affiliatedAuthor | Lee, Young Ho | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 65 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 43 | - |
dc.citation.endPage | 50 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VETERINARY MEDICAL SCIENCE, Vol.65(1) : 43-50, 2003 | - |
dc.identifier.rimsid | 55707 | - |
dc.type.rims | ART | - |
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