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생분해성 고분자 지지체의 조성과 초기 세포접종 밀도에 따른 사람 간엽 줄기세포의 골 형성 유도

Other Titles
 Osteogenic Differentiation of Human Mesenchymal Stem Cells: Effects of the Compositions of Biodegradable Polymers and Initial Cell-Seeding Density 
 김우현  ;  주소연  ;  한창동  ;  한수봉  ;  강호정 
 Journal of the Korean Orthopaedic Association (대한정형외과학회지), Vol.38(3) : 259-266, 2003 
Journal Title
 Journal of the Korean Orthopaedic Association (대한정형외과학회지) 
Issue Date
Human mesenchymal stem cells ; Osteogenic differentation ; Biodegradable polymers
Human mesenchymal stem cells ; Osteogenic differentation ; Biodegradable polymers
Purpose : To investigate the effects of initial cell-seeding density and the compositions of biodegradable polymer scaffolds on the osteogenic differentiation of human mesenchymal stem cells. Materials and Methods : Human mesenchymal stem cells (hBMSCs) were cultured three-dimensionally onto biodegradable polymers with three different compositions (65/35 PLGA, 85/15 PLGA, PLLA), and osteogenic differentiation was studied by; histology, immunohistochemistry, and transmision/scanning electron microscopy. Results : In terms of the amount of extracellular matrix (ECM) produced, the 65/35 PLGA (23% of the whole volume) was found to produce more ECM than 85/15 PLGA (21%) and PLLA (13%). Osteoblastic differentiation of hBMSCs was induced in all scaffolds examined and the deposition of ECM with calcifications started after 2 weeks of culture. The amount of calcium deposited on the 65/35 PLGA dramatically increased at 4 weeks. On comparing low cell-seeding density with the high cell-seeding density on 65/35 PLGA, calcium deposits reached similar levels at 6 weeks. Conclusion : Three-dimensional culture of hBMSCs on biodegrable polymer scaffolds induced bone formation, which was dependent on the compositions of the scaffolds and the initial cell-seeding density.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Ho Jung(강호정) ORCID logo https://orcid.org/0000-0003-0273-1264
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Hahn, Soo Bong(한수봉)
Han, Chang Dong(한창동)
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