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생분해성 고분자 지지체의 조성과 초기 세포접종 밀도에 따른 사람 간엽 줄기세포의 골 형성 유도
DC Field | Value | Language |
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dc.contributor.author | 강호정 | - |
dc.contributor.author | 김현우 | - |
dc.contributor.author | 한수봉 | - |
dc.contributor.author | 한창동 | - |
dc.date.accessioned | 2015-07-15T16:48:23Z | - |
dc.date.available | 2015-07-15T16:48:23Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1226-2102 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113635 | - |
dc.description.abstract | Purpose : To investigate the effects of initial cell-seeding density and the compositions of biodegradable polymer scaffolds on the osteogenic differentiation of human mesenchymal stem cells. Materials and Methods : Human mesenchymal stem cells (hBMSCs) were cultured three-dimensionally onto biodegradable polymers with three different compositions (65/35 PLGA, 85/15 PLGA, PLLA), and osteogenic differentiation was studied by; histology, immunohistochemistry, and transmision/scanning electron microscopy. Results : In terms of the amount of extracellular matrix (ECM) produced, the 65/35 PLGA (23% of the whole volume) was found to produce more ECM than 85/15 PLGA (21%) and PLLA (13%). Osteoblastic differentiation of hBMSCs was induced in all scaffolds examined and the deposition of ECM with calcifications started after 2 weeks of culture. The amount of calcium deposited on the 65/35 PLGA dramatically increased at 4 weeks. On comparing low cell-seeding density with the high cell-seeding density on 65/35 PLGA, calcium deposits reached similar levels at 6 weeks. Conclusion : Three-dimensional culture of hBMSCs on biodegrable polymer scaffolds induced bone formation, which was dependent on the compositions of the scaffolds and the initial cell-seeding density. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Journal of the Korean Orthopaedic Association (대한정형외과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Human mesenchymal stem cells | - |
dc.subject.MESH | Osteogenic differentation | - |
dc.subject.MESH | Biodegradable polymers | - |
dc.title | 생분해성 고분자 지지체의 조성과 초기 세포접종 밀도에 따른 사람 간엽 줄기세포의 골 형성 유도 | - |
dc.title.alternative | Osteogenic Differentiation of Human Mesenchymal Stem Cells: Effects of the Compositions of Biodegradable Polymers and Initial Cell-Seeding Density | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 김우현 | - |
dc.contributor.googleauthor | 주소연 | - |
dc.contributor.googleauthor | 한창동 | - |
dc.contributor.googleauthor | 한수봉 | - |
dc.contributor.googleauthor | 강호정 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01124 | - |
dc.contributor.localId | A00098 | - |
dc.contributor.localId | A04293 | - |
dc.contributor.localId | A04330 | - |
dc.relation.journalcode | J01839 | - |
dc.subject.keyword | Human mesenchymal stem cells | - |
dc.subject.keyword | Osteogenic differentation | - |
dc.subject.keyword | Biodegradable polymers | - |
dc.contributor.alternativeName | Kang, Ho Jung | - |
dc.contributor.alternativeName | Kim, Hyun Woo | - |
dc.contributor.alternativeName | Hahn, Soo Bong | - |
dc.contributor.alternativeName | Han, Chang Dong | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Woo | - |
dc.contributor.affiliatedAuthor | Kang, Ho Jung | - |
dc.contributor.affiliatedAuthor | Hahn, Soo Bong | - |
dc.contributor.affiliatedAuthor | Han, Chang Dong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 38 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 259 | - |
dc.citation.endPage | 266 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Orthopaedic Association (대한정형외과학회지), Vol.38(3) : 259-266, 2003 | - |
dc.identifier.rimsid | 44804 | - |
dc.type.rims | ART | - |
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