1 321

Cited 0 times in

Genome-scale analysis of resveratrol-induced gene expression profile in human ovarian cancer cells using a cDNA microarray

Authors
 Sang Hwa Yang  ;  Jong Sik Kim  ;  Sung Whan An  ;  Hyun Cheol Chung  ;  Sun Young Rha  ;  Young Ho Kim  ;  Yung Hyun Choi  ;  Hyun Sill Cho  ;  Suk Kyung Woo  ;  Sun Woo Lee  ;  Myung Soon Kim  ;  Tae Jeong Oh 
Citation
 INTERNATIONAL JOURNAL OF ONCOLOGY, Vol.22(4) : 741-750, 2003 
Journal Title
 INTERNATIONAL JOURNAL OF ONCOLOGY 
ISSN
 1019-6439 
Issue Date
2003
MeSH
Antineoplastic Agents, Phytogenic/pharmacology* ; Cell Line, Tumor ; Cell Nucleus/metabolism ; DNA, Complementary/metabolism* ; Female ; Gene Expression Regulation, Neoplastic*/drug effects* ; Genome* ; Humans ; NAD(P)H Dehydrogenase (Quinone)/metabolism ; Oligonucleotide Array Sequence Analysis ; Ovarian Neoplasms/drug therapy* ; Ovarian Neoplasms/metabolism ; RNA/metabolism ; Resveratrol ; Reverse Transcriptase Polymerase Chain Reaction ; Stilbenes/pharmacology* ; Time Factors ; Tumor Suppressor Protein p53/metabolism ; Up-Regulation
Keywords
12632063
Abstract
Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a natural compound found in large quantities, most notably in grapes and red wine, which has been shown to have anti-inflammatory, chemopreventive and anti-angiogenic effects. We examined whether resveratrol has any effect on growth and gene expression in the human ovarian cancer PA-1 cells. We show that resveratrol inhibits cell growth and induces apoptosis in PA-1 human ovarian cancer cells. We also investigated the effect of resveratrol on changes of global gene expression during resveratrol-induced growth inhibition and apoptosis in PA-1 cells using a human cDNA microarray with 7,448 sequence-verified clones. Out of the 7,448 genes screened, 118 genes were founded to be affected in their expression levels by more than 2-fold after 24-h treatment with 50 µM resveratrol. Resveratrol treatment of PA-1 cells at the final concentration of 50 µM for 6, 12, 24 and 48 h and gene expression patterns were analyzed by microarray. Clustering of the genes modulated more than 2-fold at three of the above times points divided the genes into 2 groups. Within these groups, there were specific subgroups of genes whose expressions were substantially changed at the specified time points. One of the most highly up-regulated genes found in this study was NAD(P)H quinone oxidoreductase 1(NQO-1), which has recently been shown to be involved in p53 regulation. Although the precise roles of genes whose expression levels were found to fluctuate after resveratrol treatment remain to be elucidated, we hope that the new view of gene expression in human ovarian cancer cells following resveratrol exposure, as offered by this study, provides clues for the mechanism of resveratrol action.
Full Text
http://www.spandidos-publications.com/ijo/22/4/741
DOI
10.3892/ijo.22.4.741
Appears in Collections:
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Yang, Sang Hwa(양상화)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113459
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse