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Anti-HER2/neu Peptide Producing Vector System for Biologic Therapy - Is It Possible to Mass-produce the Peptide?
DC Field | Value | Language |
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dc.contributor.author | 박병우 | - |
dc.contributor.author | 김은경 | - |
dc.date.accessioned | 2015-07-15T16:42:49Z | - |
dc.date.available | 2015-07-15T16:42:49Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113449 | - |
dc.description.abstract | A humanized monoclonal antibody against HER2 has been using in a clinical setting and has been shown to possess therapeutic properties. A mimetic peptide against HER2 was also reported to bind to the HER2 receptor with some therapeutic potential. Based on a previous report and the sequence of Herceptin, we designed oligonucleotides of anti-HER2 mimetic peptides, named V2 and V3 peptides, in order to develop a peptide-producing vector system for biologic therapy against HER2-overexpressing cancers. We also adopted the sequence of a previously reported mimetic peptide, V1 (Park BW et al. Nat. Biotechnol, 2000, 18: 194-198), as a reference peptide. We examined the effects of the V2 and V3 peptides against the HER2-overexpressing cell lines, SK-BR-3 and T6-17. Transient transfection of the construct expressing V1 and V2 inhibited cell proliferation in HER2-overexpressing cell lines by 20 - 30%, but had no effect on the HER2-negative NIH3T3 cells. The proliferation inhibition shown by V2 was slightly better than that shown by V1. Recombinant peptides V2 and V3 were produced on a large scale in an E. coli system, and the V2 peptide showed anti-HER2-specific tumor cell proliferation inhibition of 10% to 30%. Current results suggest that anti-HER2 mimetic peptides, overexpressed by a constitutive promoter or produced in an E. coli system, could specifically inhibit the proliferation of HER2-expressing cancer cells. Further efforts to augment the biologic specificity and efficacy and to develop new technologies for the purification of the peptide from the E coli system are needed. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 58~64 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Division/drug effects | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Oligopeptides/chemical synthesis | - |
dc.subject.MESH | Oligopeptides/pharmacology | - |
dc.subject.MESH | Peptide Fragments/chemical synthesis* | - |
dc.subject.MESH | Peptide Fragments/pharmacology* | - |
dc.subject.MESH | Receptor, ErbB-2/chemistry* | - |
dc.subject.MESH | Recombinant Proteins/chemical synthesis | - |
dc.subject.MESH | Recombinant Proteins/pharmacology | - |
dc.subject.MESH | Technology, Pharmaceutical* | - |
dc.subject.MESH | Transfection | - |
dc.title | Anti-HER2/neu Peptide Producing Vector System for Biologic Therapy - Is It Possible to Mass-produce the Peptide? | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Byeong Woo Park | - |
dc.contributor.googleauthor | Ki Suk Kim | - |
dc.contributor.googleauthor | Kyung Sup Kim | - |
dc.contributor.googleauthor | Eun Kyung Kim | - |
dc.contributor.googleauthor | Kyong Sik Lee | - |
dc.contributor.googleauthor | Min Kyu Heo | - |
dc.identifier.doi | 10.3349/ymj.2003.44.1.58 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01475 | - |
dc.contributor.localId | A00801 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 12619176 | - |
dc.subject.keyword | HER2/neu | - |
dc.subject.keyword | mimetic peptide | - |
dc.subject.keyword | biologic therapy | - |
dc.contributor.alternativeName | Park, Byeong Woo | - |
dc.contributor.alternativeName | Kim, Eun Kyung | - |
dc.contributor.affiliatedAuthor | Park, Byeong Woo | - |
dc.contributor.affiliatedAuthor | Kim, Eun-Kyung | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 44 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 58 | - |
dc.citation.endPage | 64 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.44(1) : 58-64, 2003 | - |
dc.identifier.rimsid | 49272 | - |
dc.type.rims | ART | - |
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